De Novo Design of a Single-Chain Diphenylporphyrin Metalloprotein

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dc.contributor.authorBender, Gretchen M.
dc.contributor.authorLehmann, Andreas
dc.contributor.authorZou, Hongling
dc.contributor.authorCheng, Hong
dc.contributor.authorFry, H. Christopher
dc.contributor.authorEngel, Don
dc.contributor.authorTherien, Michael J.
dc.contributor.authorBlasie, J. Kent
dc.contributor.authorRoder, Heinrich
dc.contributor.authorSaven, Jeffrey G.
dc.contributor.authorDeGrado, William F.
dc.date.accessioned2021-04-30T15:31:18Z
dc.date.available2021-04-30T15:31:18Z
dc.date.issued2007-08-10
dc.description.abstractWe describe the computational design of a single-chain four-helix bundle that noncovalently self-assembles with fully synthetic non-natural porphyrin cofactors. With this strategy, both the electronic structure of the cofactor as well as its protein environment may be varied to explore and modulate the functional and photophysical properties of the assembly. Solution characterization (NMR, UV−vis) of the protein showed that it bound with high specificity to the desired cofactors, suggesting that a uniquely structured protein and well-defined site had indeed been created. This provides a genetically expressed single-chain protein scaffold that will allow highly facile, flexible, and asymmetric variations to enable selective incorporation of different cofactors, surface-immobilization, and introduction of spectroscopic probes.en_US
dc.description.sponsorshipThis work was supported by the DOE grant DE-FG02-04ER46156 (MJT, JGS, WFD) and the National Science Foundation grant NSEC DMR-0425780. Partial support was also provided by the NIH Grants GM54616 to W.F.D., P0148130 to J.G.S., and GM071628 to M.J.T. We also thank James Lear for interpreting the AUC data.en_US
dc.description.urihttps://pubs.acs.org/doi/10.1021/ja071199jen_US
dc.format.extent20 pagesen_US
dc.genrejournal articles preprintsen_US
dc.identifierdoi:10.13016/m2yj0b-gpyg
dc.identifier.citationBender et al., De Novo Design of a Single-Chain Diphenylporphyrin Metalloprotein, J Am Chem Soc. 2007 September 5; 129(35): 10732–10740. doi:10.1021/ja071199j.en_US
dc.identifier.urihttps://doi.org/10.1021/ja071199j
dc.identifier.urihttp://hdl.handle.net/11603/21409
dc.language.isoen_USen_US
dc.publisherACS Publicationsen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Computer Science and Electrical Engineering Department Collection
dc.relation.ispartofUMBC Office for the Vice President of Research
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsThis document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Journal of the American Chemical Society, copyright © American Chemical Society after peer review. To access the final edited and published work see https://doi.org/10.1021/ja071199j.
dc.titleDe Novo Design of a Single-Chain Diphenylporphyrin Metalloproteinen_US
dc.typeTexten_US

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