Characterization of protein release from hydrolytically degradable poly(ethylene glycol) hydrogels
| dc.contributor.author | Zustiak, Silviya P. | |
| dc.contributor.author | Leach, Jennie B. | |
| dc.date.accessioned | 2018-12-17T16:32:25Z | |
| dc.date.available | 2018-12-17T16:32:25Z | |
| dc.date.issued | 2010-08-27 | |
| dc.description.abstract | We present a novel fully hydrophilic, hydrolytically degradable poly(ethylene glycol) (PEG) hydrogel suitable for soft tissue engineering and delivery of protein drugs. The gels were designed to overcome drawbacks associated with current PEG hydrogels (i.e., reaction mechanisms or degradation products that compromise protein stability): the highly selective and mild cross‐linking reaction allowed for encapsulating proteins prior to gelation without altering their secondary structure as shown by circular dichroism experiments. Further, hydrogel degradation and structure, represented by mesh size, were correlated to protein release. It was determined that polymer density had the most profound effect on protein diffusivity, followed by the polymer molecular weight, and finally by the specific chemical structure of the cross‐linker. By examining the diffusion of several model proteins, we confirmed that the protein diffusivity was dependent on protein size as smaller proteins (e.g., lysozyme) diffused faster than larger proteins (e.g., Ig). Furthermore, we demonstrated that the protein physical state was preserved upon encapsulation and subsequent release from the PEG hydrogels and contained negligible aggregation or protein–polymer adducts. These initial studies indicate that the developed PEG hydrogels are suitable for release of stable proteins in drug delivery and tissue engineering applications. | en_US |
| dc.description.sponsorship | This work was supported by NIH‐NINDS (R01NS065205), the Henry Luce Foundation, and UMBC. | en_US |
| dc.description.uri | https://onlinelibrary.wiley.com/doi/full/10.1002/bit.22911 | en_US |
| dc.format.extent | 10 pages | en_US |
| dc.genre | journal articles postprints | en_US |
| dc.identifier | doi:10.13016/M25X25H5G | |
| dc.identifier.citation | Silviya P. Zustiak, Jennie B. Leach, Characterization of protein release from hydrolytically degradable poly(ethylene glycol) hydrogels, Biotechnology and Bioengineering, Volume 108, Issue 1 , 2010, https://doi.org/10.1002/bit.22911 | en_US |
| dc.identifier.uri | https://doi.org/10.1002/bit.22911 | |
| dc.identifier.uri | http://hdl.handle.net/11603/12280 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Wiley Periodicals | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Chemical, Biochemical & Environmental Engineering Department Collection | |
| dc.relation.ispartof | UMBC Faculty Collection | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | "This is the peer reviewed version of the following article: Silviya P. Zustiak, Jennie B. Leach, Characterization of protein release from hydrolytically degradable poly(ethylene glycol) hydrogels, Biotechnology and Bioengineering, Volume 108, Issue 1 , 2010, https://doi.org/10.1002/bit.22911, which has been published in final form at https://doi.org/10.1002/bit.22911. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions." | |
| dc.subject | protein | en_US |
| dc.subject | diffusion | en_US |
| dc.subject | hydrolytic degradation | en_US |
| dc.subject | PEG hydrogel | en_US |
| dc.subject | BSA | en_US |
| dc.title | Characterization of protein release from hydrolytically degradable poly(ethylene glycol) hydrogels | en_US |
| dc.type | Text | en_US |