Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant

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dc.contributor.authorSchreier, Harold J.
dc.contributor.authorDejtisakdi, Wipawee
dc.contributor.authorEscalante, Jose Ortega
dc.contributor.authorBrailo, Marina
dc.date.accessioned2019-03-22T15:17:53Z
dc.date.available2019-03-22T15:17:53Z
dc.date.issued2012-10
dc.description.abstractA Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus.en_US
dc.description.sponsorshipThis work was supported, in part, by a grant from the United States- Israel Binational Agricultural Research and Development Fund (MB- 8720-08).en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457482/en_US
dc.format.extent4 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2l2uo-1fev
dc.identifier.citationHarold J. Schreier, Wipawee Dejtisakdi, Jose Ortega Escalante, and Marina Brailo, Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant, Appl Environ Microbiol. 2012 Oct; 78(19): 7120–7123, DOI: 10.1128/AEM.01794-12en_US
dc.identifier.urihttps://doi.org/10.1128/AEM.01794-12
dc.identifier.urihttp://hdl.handle.net/11603/13127
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Department of Marine Biotechnology
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Biological Sciences Department
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectPlanctomyces limnophilusen_US
dc.subjectEZ-Tn5 transposomeen_US
dc.subjectphosphoenolpyruvate carboxykinaseen_US
dc.titleTransposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutanten_US
dc.typeTexten_US

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