Taste Receptor Cell Responses to the Bitter Stimulus Denatonium Involve Ca2+ Influx Via Store-Operated Channels

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dc.contributor.authorOgura, Tatsuya
dc.contributor.authorMargolskee, Robert F.
dc.contributor.authorKinnamon, Sue C.
dc.date.accessioned2021-02-23T18:48:57Z
dc.date.available2021-02-23T18:48:57Z
dc.date.issued2002-06-01
dc.descriptionUMBC Weihong Lin Lab
dc.description.abstractPrevious studies in rat and mouse have shown that brief exposure to the bitter stimulus denatonium induces an increase in [Ca2+]i due to Ca2+ release from intracellular Ca2+ stores, rather than Ca2+influx. We report here that prolonged exposure to denatonium induces sustained increases in [Ca2+]i that are dependent on Ca2+ influx. Similar results were obtained from taste cells of the mudpuppy, Necturus maculosus, as well as green fluorescent protein (GFP) tagged gustducin-expressing taste cells of transgenic mice. In a subset of mudpuppy taste cells, prolonged exposure to denatonium induced oscillatory Ca2+responses. Depletion of Ca2+ stores by thapsigargin also induced Ca2+ influx, suggesting that Ca2+store-operated channels (SOCs) are present in both mudpuppy taste cells and gustducin-expressing taste cells of mouse. Further, treatment with thapsigargin prevented subsequent responses to denatonium, suggesting that the SOCs were the source of the Ca2+ influx. These data suggest that SOCs may contribute to bitter taste transduction and to regulation of Ca2+ homeostasis in taste cells.en_US
dc.description.sponsorshipR. F. Margolskee is an Associate Investigator of the Howard Hughes Medical Institute. This work was supported by National Institute on Deafness and Other Communication Disorders Grants DC-00766 and DC-00244 to S. C. Kinnamon and DC-03055 and DC-03155 to R. F. Margolskee.en_US
dc.description.urihttps://journals.physiology.org/doi/full/10.1152/jn.2002.87.6.3152en_US
dc.format.extent4 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2pknt-r38g
dc.identifier.citationTatsuya Ogura, Robert F. Margolskee, and Sue C. Kinnamon, Taste Receptor Cell Responses to the Bitter Stimulus Denatonium Involve Ca2+ Influx Via Store-Operated Channels, . J Neurophysiol 87: 3152–3155, 2002; 10.1152/jn.00780.2001.en_US
dc.identifier.urihttps://doi.org/10.1152/jn.2002.87.6.3152
dc.identifier.urihttp://hdl.handle.net/11603/21072
dc.language.isoen_USen_US
dc.publisherAmerican Physiological Society (APS)en_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjecttaste receptor cellsen_US
dc.subjectbitter stimulusen_US
dc.subjectdenatoniumen_US
dc.subjectmudpuppyen_US
dc.titleTaste Receptor Cell Responses to the Bitter Stimulus Denatonium Involve Ca2+ Influx Via Store-Operated Channelsen_US
dc.typeTexten_US

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