A green nonsulfur bacterium, Dehalococcoides ethenogenes, with the LexA binding sequence found in gram-positive organisms

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dc.contributor.authorHenestrosa, Antonio R. Fernández de
dc.contributor.authorCuñé, Jordi
dc.contributor.authorErill, Ivan
dc.contributor.authorMagnuson, Jon K.
dc.contributor.authorBarbé, Jordi
dc.date.accessioned2021-03-10T16:59:55Z
dc.date.available2021-03-10T16:59:55Z
dc.date.issued2002-11
dc.description.abstractDehalococcoides ethenogenes is a member of the physiologically diverse division of green nonsulfur bacteria. Using a TBLASTN search, the D. ethenogenes lexA gene has been identified, cloned, and expressed and its protein has been purified. Mobility shift assays revealed that the D. ethenogenes LexA protein specifically binds to both its own promoter and that of the uvrA gene, but not to the recA promoter. Our results demonstrate that the D. ethenogenes LexA binding site is GAACN4GTTC, which is identical to that found in gram-positive bacteria. In agreement with this fact, the Bacillus subtilis DinR protein binds specifically to the D. ethenogenes LexA operator. This constitutes the first non-gram-positive bacterium exhibiting a LexA binding site identical to that of B. subtilis.en_US
dc.description.sponsorshipThis work was funded by grants BMC2001-2065 from the Ministerio de Ciencia y Tecnología (MCyT) de España and 2001SGR-206 from the Departament d'Universitats, Recerca i Societat de la Informació (DURSI) de la Generalitat de Catalunya. Antonio R. Fernández de Henestrosa is a recipient of a postdoctoral reincorporation contract from the MCyT. We acknowledge Joan Ruiz, Susana Escribano, and Pilar Cortés for excellent technical assistance. Preliminary D. ethenogenes sequence data were obtained from the Institute for Genomic Research website at http://www.tigr.org . The Chlorefexus aurantiacus partial lexA sequence was provided free by the U.S.-DOE Joint Genome Institute (http://www.jgi.doe.gov/ ). We are deeply grateful to Roger Woodgate and Kevin Winterling for generously providing us with B. subtilis genomic DNA, B. subtilis DinR, and E. coli LexA protein.en_US
dc.description.urihttps://jb.asm.org/content/184/21/6073.longen_US
dc.format.extent8 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2zvjp-vuep
dc.identifier.citationAntonio R. Fernández de Henestrosa, Jordi Cuñé, Ivan Erill, Jon K. Magnuson, Jordi Barbé, A Green Nonsulfur Bacterium, Dehalococcoides ethenogenes, with the LexA Binding Sequence Found in Gram-Positive Organisms,Journal of Bacteriology Nov 2002, 184 (21) 6073-6080; DOI: 10.1128/JB.184.21.6073-6080.2002en_US
dc.identifier.urihttp://dx.doi.org/10.1128/JB.184.21.6073-6080.2002
dc.identifier.urihttp://hdl.handle.net/11603/21136
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectDehalococcoides ethenogenesen_US
dc.subjectTBLASTN searchen_US
dc.subjectD. ethenogenes LexA binding siteen_US
dc.subjectGAACN4GTTCen_US
dc.titleA green nonsulfur bacterium, Dehalococcoides ethenogenes, with the LexA binding sequence found in gram-positive organismsen_US
dc.typeTexten_US

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