Engineering of a Stable Collagen Nanofibrous Scaffold with Tunable Fiber Diameter, Alignment, and Mechanical Properties
Author/Creator ORCID
Date
Type of Work
Department
Program
Citation of Original Publication
Castilla-Casadiego, David A., Heleine V. Ramos-Avilez, Stephany Herrera-Posada, Barbara Calcagno, Luis Loyo, Jacoby Shipmon, Aldo Acevedo, Anibal Quintana, and Jorge Almodovar. “Engineering of a Stable Collagen Nanofibrous Scaffold with Tunable Fiber Diameter, Alignment, and Mechanical Properties.” Macromolecular Materials and Engineering 301, no. 9 (June 15, 2016): 1064–75. https://doi.org/10.1002/mame.201600156.
Rights
This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
Abstract
The effect of varying electrospinning parameters is reported for the production of collagen nanofibers from acetic acid with controlled fiber diameter, orientation, and mechanical properties. Nanofibers with a range of diameters of 175–400 nm are obtained by varying either the voltage or the flow rate. An increase in nanofiber alignment is observed by increasing injection flow rate. Mechanical testing of these fibers reveals that the elasticity modulus can be tuned in the range of 2.7–4.1 MPa by the selection of the crosslinking method. Fourier transform infrared spectroscopy reveals that the secondary structure of collagen is preserved after electrospinning and crosslinking. Lastly, in vitro testing reveals that a high number of fibroblasts attach to the collagen matrices indicating, that they are suitable for mammalian cell culture.