Bioimaging system using acousto-optic tunable filter

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https://www.spiedigitallibrary.org/conference-proceedings-of-spie/3911/0000/Bioimaging-system-using-acousto-optic-tunable-filter/10.1117/12.384922.full

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https://doi.org/10.1117/12.384922
 http://hdl.handle.net/11603/36058

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UMBC Chemistry & Biochemistry Department
UMBC Faculty Collection

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https://orcid.org/0000-0002-5250-8290

Date

2000-05-03

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conference papers and proceedings
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Citation of Original Publication

Kasili, Paul M., Joel Mobley, Brian M. Cullum, and Tuan Vo-Dinh. “Bioimaging System Using Acousto-Optic Tunable Filter.” In Biomedical Diagnostic, Guidance, and Surgical-Assist Systems II, 3911. (May 3, 2000):345–54. https://doi.org/10.1117/12.384922.

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This work was written as part of one of the author's official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.
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Abstract

The interaction of light with tissue has ben used to recognize disease since the mid-1800s. The recent developments of light sources, detectors, and fiber optic probes provide opportunities to measure these interactions, which yield information for tissue diagnosis at the biochemical, structural, or physiological level. In this paper, we describe a bioimaging system designed for biomedical applications and show laser-indued fluorescence (LIF) images mammalian brain tissue. The LIF imaging of tissue was carried out in vitro using two laser excitations: 488 nm and 514 nm. Images were recorded through an acousto- optic tunable filter over the range 500 nm-650 nm with a charged coupled device camera. Background subtracted images were generated across the fluorescent wavelength. Subtraction allowed a safe comparison to be made with well- contrasted images. Of the two tested excitation wavelengths, 488 nm excitation gave the more distinctive contrast.