Identification of the Acidobacterium capsulatum LexA box reveals a lateral acquisition of the Alphaproteobacteria lexA gene

Simple item page
dc.contributor.authorMazón, Gerard
dc.contributor.authorCampoy, Susana
dc.contributor.authorErill, Ivan
dc.contributor.authorBarbé, Jordi
dc.date.accessioned2021-03-09T17:18:03Z
dc.date.available2021-03-09T17:18:03Z
dc.date.issued2006-04-01
dc.description.abstractAcidobacterium capsulatum is the most thoroughly studied species of a new bacterial phylogenetic group designated the phylum Acidobacteria. Through a tblastn search, the A. capsulatum lexA gene has been identified, and its product purified. Electrophoretic mobility shift assays have shown that A. capsulatum LexA protein binds specifically to the direct repeat GTTCN7GTTC motif. Strikingly, this is also the LexA box of the Alphaproteobacteria, but had not previously been described outside this subclass of the Proteobacteria. In addition, a phylogenetic analysis of the LexA protein clusters together Acidobacterium and the Alphaproteobacteria, moving the latter away from their established phylogenetic position as a subclass of the Proteobacteria, and pointing to a lateral gene transfer of the lexA gene from the phylum Acidobacteria, or an immediate ancestor, to the Alphaproteobacteria. Lastly, in vivo experiments demonstrate that the A. capsulatum recA gene is DNA-damage inducible, despite the fact that a LexA-binding sequence is not present in its promoter region.en_US
dc.description.sponsorshipThis work was funded by grant BFM2004-02768/BMC from the Ministerio de Educacio´n y Ciencia (MEC) de Espan˜a, and by the Consejo Superior de Investigaciones Cientı´ficas (CSIC). S. C. is a recipient of a postdoctoral contract from Instituto Nacional de Investigaciones y Tecnologı´a Agraria y Alimentaria (INIA) – Institute Recerca i Tecnologia Agroalimenta`ries (IRTA). We are deeply grateful to Joan Ruiz and Dr Pilar Corte´s for their excellent technical assistance.en_US
dc.description.urihttps://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.28376-0#tab2en_US
dc.format.extent10 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m26ea8-eikb
dc.identifier.citationGerard Mazón, Susana Campoy, Ivan Erill and Jordi Barbé, Identification of the Acidobacterium capsulatum LexA box reveals a lateral acquisition of the Alphaproteobacteria lexA gene,Microbiology (2006), 152, 1109–1118, DOI 10.1099/mic.0.28376-0en_US
dc.identifier.urihttps://doi.org/10.1099/mic.0.28376-0
dc.identifier.urihttp://hdl.handle.net/11603/21114
dc.language.isoen_USen_US
dc.publisherMicrobiology Societyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectEMSAen_US
dc.subjectelectrophoretic mobility shift assayen_US
dc.subjectLGTen_US
dc.subjectlateral gene transferen_US
dc.titleIdentification of the Acidobacterium capsulatum LexA box reveals a lateral acquisition of the Alphaproteobacteria lexA geneen_US
dc.typeTexten_US

Files

License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.56 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UMBC Biological Sciences Department