Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay

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dc.contributor.authorCampbell, Shonda M.
dc.contributor.authorStarz-Gaiano, Michelle
dc.contributor.authorLeips, Jeff
dc.date.accessioned2020-08-06T16:02:45Z
dc.date.available2020-08-06T16:02:45Z
dc.date.issued2020-06-11
dc.description.abstractPhagocytosis is an essential function of the innate immune response. This process is carried out by phagocytic hemocytes whose primary function is to recognize a wide range of particles and destroy microbial pathogens. As organisms age, this process begins to decline, yet little is known about the underlying mechanisms or the genetic basis of immunosenescence. Here, an injection based in vivo phagocytosis assay is used to assess age related changes in different aspects of phagocytosis, such as binding, engulfment, and degradation of internalized particles, by quantifying phagocytic events in hemocytes in adult Drosophila. Drosophila melanogaster has become an ideal model to investigate age related changes in innate immune function for many reasons. For one, many genetic components and functions of the innate immune response, including phagocytosis, are evolutionarily conserved between Drosophila and mammals. Because of that, results obtained from using this protocol are likely to be widely relevant to understanding the age related changes in immune function in a variety of organisms. Additionally, we note that this method provides quantitative estimates of hemocyte phagocytic ability, which could be useful for a variety of research topics, and need not be limited to studies of aging.en_US
dc.description.sponsorshipThis work was supported by grants from the National Institutes of Health R03 AG061484-02 and the UMBC College of Natural and Mathematical Sciences Becton Dickinson Faculty Research Fund.en_US
dc.description.urihttps://www.jove.com/t/60983/assessing-age-specific-phagocytic-ability-adult-drosophilaen_US
dc.format.extent16 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2yhe8-drbz
dc.identifier.citationCampbell, Shonda M.; Starz-Gaiano, Michelle; Leips, Jeff; Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay; Journal of Visualized Experiments (160), e60983 (2020); https://www.jove.com/t/60983/assessing-age-specific-phagocytic-ability-adult-drosophilaen_US
dc.identifier.urihttps://dx.doi.org/10.3791/60983
dc.identifier.urihttp://hdl.handle.net/11603/19348
dc.language.isoen_USen_US
dc.publisherJOVEen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Student Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0)*
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/*
dc.titleAssessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assayen_US
dc.typeTexten_US

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