Newcastle Disease Virus Vectored Chicken Infectious Anaemia Vaccine Induces Robust Immune Response in Chickens

Simple item page
dc.contributor.authorChellappa, Madhan Mohan
dc.contributor.authorDey, Sohini
dc.contributor.authorPathak, Dinesh Chandra
dc.contributor.authorSingh, Asmita
dc.contributor.authorRamamurthy, Narayan
dc.contributor.authorRamakrishnan, Saravanan
dc.contributor.authorMariappan, Asok Kumar
dc.contributor.authorDhama, Kuldeep
dc.contributor.authorVakharia, Vikram
dc.date.accessioned2021-11-03T18:00:59Z
dc.date.available2021-11-03T18:00:59Z
dc.date.issued2021-10-02
dc.description.abstractNewcastle disease virus (NDV) strain R2B, with an altered fusion protein cleavage site, was used as a viral vector to deliver the immunogenic genes VP2 and VP1 of chicken infectious anaemia virus (CIAV) to generate a bivalent vaccine candidate against these diseases in chickens. The immunogenic genes of CIAV were expressed as a single transcriptional unit from the NDV backbone and the two CIA viral proteins were obtained as separate entities using a self-cleaving foot-and-mouth disease virus 2A protease sequence between them. The recombinant virus (rR2B-FPCS-CAV) had similar growth kinetics as that of the parent recombinant virus (rR2B-FPCS) in vitro with similar pathogenicity characteristics. The bivalent vaccine candidate when given in specific pathogen-free chickens as primary and booster doses was able to elicit robust humoral and cell-mediated immune (CMI) responses obtained in a vaccination study that was conducted over a period of 15 weeks. In an NDV and CIAV ELISA trial, there was a significant difference in the titres of antibody between vaccinated and control groups which showed slight reduction in antibody titre by 56 days of age. Hence, a second booster was administered and the antibody titres were maintained until 84 days of age. Similar trends were noticed in CMI response carried out by lymphocyte transformation test, CD4+ and CD8+ response by flow cytometry analysis and response of real time PCR analysis of cytokine genes. Birds were challenged with virulent NDV and CIAV at 84 days and there was significant reduction in the NDV shed on the 2nd and 4th days post challenge in vaccinated birds as compared to unvaccinated controls. Haematological parameters comprising PCV, TLC, PLC and PHC were estimated in birds that were challenged with CIAV that indicated a significant reduction in the blood parameters of controls. Our findings support the development and assessment of a bivalent vaccine candidate against NDV and CIAV in chickens.en_US
dc.description.sponsorshipThe research work was supported by grant from the Department of Biotechnology, Government of India (BT/PR21576/ADV/90/249/2016 to MMC).en_US
dc.description.urihttps://www.mdpi.com/1999-4915/13/10/1985en_US
dc.format.extent20 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m21mgj-3h9n
dc.identifier.citationChellappa, Madhan Mohan et al.; Newcastle Disease Virus Vectored Chicken Infectious Anaemia Vaccine Induces Robust Immune Response in Chickens; Viruses, 13(10), 1985, 2 October, 2021; https://doi.org/10.3390/v13101985en_US
dc.identifier.urihttps://doi.org/10.3390/v13101985
dc.identifier.urihttp://hdl.handle.net/11603/23200
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Department of Marine Biotechnology
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.titleNewcastle Disease Virus Vectored Chicken Infectious Anaemia Vaccine Induces Robust Immune Response in Chickensen_US
dc.typeTexten_US
dcterms.creatorhttps://orcid.org/0000-0002-0955-3010en_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
viruses-13-01985-v3.pdf
Size:
7.85 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.56 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UMBC Department of Marine Biotechnology
UMBC Faculty Collection