Identification and characterization of the pckA gene from Staphylococcus aureus

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC178095/

Permanent Link

http://hdl.handle.net/11603/13077

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UMBC Biological Sciences Department
UMBC Department of Marine Biotechnology
UMBC Faculty Collection

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Date

1996-06

Type of Work

journal articles
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Citation of Original Publication

W. H. Scovill, H. J. Schreier, and K. W. Bayles, Identification and characterization of the pckA gene from Staphylococcus aureus, J Bacteriol. 1996 Jun; 178(11): 3362–3364, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC178095/

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Subjects

Staphylococcus aureus
pckA gene
phosphoenolpyruvate carboxykinase

Abstract

The Staphylococcus aureus pckA gene was identified and characterized. A pckA mutant lacked detectable phosphoenolpyruvate carboxykinase activity and grew poorly in the absence of glucose. Both enzymatic activity and pckA promoter activity in wild-type cells grown in the absence of glucose were at least 22-fold greater than activities in cells grown in the presence of glucose.