Engineering angiogenesis following spinal cord injury: a coculture of neural progenitor and endothelial cells in a degradable polymer implant leads to an increase in vessel density and formation of the blood–spinal cord barrier

dc.contributor.authorRauch, Millicent Ford
dc.contributor.authorHynes, Sara Royce
dc.contributor.authorBertram, James
dc.contributor.authorRedmond, Andy
dc.contributor.authorRobinson, Rebecca
dc.contributor.authorWilliams, Cicely
dc.contributor.authorXu, Hao
dc.contributor.authorMadri, Joseph A.
dc.contributor.authorLavik, Erin
dc.date.accessioned2021-03-19T18:08:44Z
dc.date.available2021-03-19T18:08:44Z
dc.date.issued2008-12-22
dc.description.abstractAngiogenesis precedes recovery following spinal cord injury and its extent correlates with neural regeneration, suggesting that angiogenesis may play a role in repair. An important precondition for studying the role of angiogenesis is the ability to induce it in a controlled manner. Previously, we showed that a coculture of endothelial cells (ECs) and neural progenitor cells (NPCs) promoted the formation of stable tubes in vitro and stable, functional vascular networks in vivo in a subcutaneous model. We sought to test whether a similar coculture would lead to the formation of stable functional vessels in the spinal cord following injury. We created microvascular networks in a biodegradable two‐component implant system and tested the ability of the coculture or controls (lesion control, implant alone, implant + ECs or implant + NPCs) to promote angiogenesis in a rat hemisection model of spinal cord injury. The coculture implant led to a fourfold increase in functional vessels compared with the lesion control, implant alone or implant + NPCs groups and a twofold increase in functional vessels over the implant + ECs group. Furthermore, half of the vessels in the coculture implant exhibited positive staining for the endothelial barrier antigen, a marker for the formation of the blood–spinal cord barrier. No other groups have shown positive staining for the blood–spinal cord barrier in the injury epicenter. This work provides a novel method to induce angiogenesis following spinal cord injury and a foundation for studying its role in repair.en_US
dc.description.sponsorshipThis work was funded through the generous support of Richard and Gail Siegal, the Discovery Eye Foundation, and the Lincy Foundation. MFR, JB, and RR acknowledge a NIH Neuroengineering Training Grant, NIH T90-DK070068. MFR would like to sincerely thank the NINDS Ohio State's Spinal Cord Injury Training Course for teaching her the spinal cord surgeries and behavioral techniques. We'd like to thank Rosh and Roshan Sethi for help with animal care. Many thanks to Dr. John Kiernan for helpful advice in eriochrome cyanine staining. We are also grateful for Dr. Jeffrey Kocsis for his patient consultations and suggestions.en_US
dc.description.urihttps://onlinelibrary.wiley.com/doi/abs/10.1111/j.1460-9568.2008.06567.xen_US
dc.format.extent23 pagesen_US
dc.genrejournal articles postprintsen_US
dc.identifierdoi:10.13016/m2mhr2-bbqs
dc.identifier.citationMillicent Ford Rauch, Sara Royce Hynes et al., Engineering angiogenesis following spinal cord injury: a coculture of neural progenitor and endothelial cells in a degradable polymer implant leads to an increase in vessel density and formation of the blood–spinal cord barrier, Eur J Neurosci. 2009 January ; 29(1): 132–145. doi:10.1111/j.1460-9568.2008.06567.x.en_US
dc.identifier.urihttps://doi.org/10.1111/j.1460-9568.2008.06567.x
dc.identifier.urihttp://hdl.handle.net/11603/21200
dc.language.isoen_USen_US
dc.publisherWileyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsThis is the peer reviewed version of the following article: Millicent Ford Rauch Sara Royce Hyneset al., Engineering angiogenesis following spinal cord injury: a coculture of neural progenitor and endothelial cells in a degradable polymer implant leads to an increase in vessel density and formation of the blood–spinal cord barrier, Eur J Neurosci. 2009 January ; 29(1): 132–145. doi:10.1111/j.1460-9568.2008.06567.x, which has been published in final form at https://doi.org/10.1111/j.1460-9568.2008.06567.x. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
dc.titleEngineering angiogenesis following spinal cord injury: a coculture of neural progenitor and endothelial cells in a degradable polymer implant leads to an increase in vessel density and formation of the blood–spinal cord barrieren_US
dc.typeTexten_US

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