Qualitative and Quantitative Analysis of the Corneal Endothelium With Smartphone Specular Microscopy
| dc.contributor.author | Fliotsos, Michael J. | |
| dc.contributor.author | Deljookorani, Shiva | |
| dc.contributor.author | Dzhaber, Daliya | |
| dc.contributor.author | Chandan, Subhangi | |
| dc.contributor.author | Ighani, Mehrnaz | |
| dc.contributor.author | Eghrari, Allen O. | |
| dc.date.accessioned | 2020-03-25T16:51:35Z | |
| dc.date.available | 2020-03-25T16:51:35Z | |
| dc.date.issued | 2020-02-14 | |
| dc.description.abstract | Purpose: We sought to demonstrate the feasibility of a lower-cost, portable method for qualitative and quantitative analysis of the corneal endothelium using a smartphone and slit lamp biomicroscope. Methods: In this study at a single academic center, we recruited healthy participants to undergo imaging of the corneal endothelium using both a smartphone-based method and a specular microscope. Participants first had their eyes imaged with a CellChek® NSP-9900 Specular Microscope (Konan TM Medical, Inc.; Irvine, CA, USA). For image capture on the smartphone, a beam of light approximately 0.2mm in diameter was directed to the center of the cornea with a slit-lamp biomicroscope to achieve specular reflection. With 40x zoom on the slit-lamp and 4K video mode set on an iPhone 7 Plus held to an ocular, the corneal endothelium was recorded until the hexagonal pattern of cells was identified, and the sharpest frame from the video selected. Results: Videos were analyzed from 14 sets of eyes (average length 2 minutes 40 seconds). The average intraclass correlation coefficient was 0.67 (95% CI 0.43-0.82). The mean difference between smartphone endothelial cell count and specular endothelial cell count was -209 cells/mm² (standard deviation = 483 cells/mm²), which did not achieve significance (p=0.14). Bland-Altman analysis with simple linear regression showed no proportional bias when comparing the two modalities (coefficient =-0.20; t-value = -0.42; P = 0.68). Conclusion: Smartphone specular microscopy is capable of qualitative and quantitative analysis of the corneal endothelium. Further refinement to standardize the light source and automate analysis will increase feasibility. | en_US |
| dc.description.sponsorship | We would like to thank the David and Cynthia Tolsma Fund for their generosity in supporting this work, the Special Scholar Award from Research to Prevent Blindness, and our volunteer subjects for their gracious involvement. | |
| dc.description.uri | https://journals.lww.com/corneajrnl/Abstract/publishahead/Qualitative_and_Quantitative_Analysis_of_the.96212.aspx | en_US |
| dc.format.extent | 17 pages | en_US |
| dc.genre | journal articles postprints | en_US |
| dc.identifier | doi:10.13016/m2hg18-8vpg | |
| dc.identifier.citation | Fliotsos, Michael J.; Deljookorani, Shiva; Dzhaber, Daliya; Chandan, Subhangi; Ighani, Mehrnaz; Eghrari, Allen O.; Qualitative and Quantitative Analysis of the Corneal Endothelium With Smartphone Specular Microscopy; The Journal of Cornea and External Disease (2020); https://journals.lww.com/corneajrnl/Abstract/publishahead/Qualitative_and_Quantitative_Analysis_of_the.96212.aspx | en_US |
| dc.identifier.uri | https://doi.org/10.1097/ICO.0000000000002277 | |
| dc.identifier.uri | http://hdl.handle.net/11603/17640 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Wolters Kluwer | |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Biological Sciences Department Collection | |
| dc.relation.ispartof | UMBC Student Collection | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | Access to this item will begin on 2021-02-14 | |
| dc.rights | This is not the final published version. | |
| dc.title | Qualitative and Quantitative Analysis of the Corneal Endothelium With Smartphone Specular Microscopy | en_US |
| dc.type | Text | en_US |