High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli

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dc.contributor.authorZengel, J. M.
dc.contributor.authorLindahl, L.
dc.date.accessioned2019-06-04T18:07:50Z
dc.date.available2019-06-04T18:07:50Z
dc.date.issued1981-01
dc.description.abstractWe have constructed a high-copy-number plasmid carrying an allele of the supD gene (supD43,74). The plasmid conferred temperature-sensitive suppression of amber mutations. Strains carrying the plasmid exhibited 50 to 60% suppression at 30 degrees C but little or no suppression at 42 degrees C. After a temperature shift from 30 to 42 degrees C the efficiency of suppression decreased gradually over a 60- to 90-min period before reaching the 42 degrees C steady-state level of suppression.en
dc.description.sponsorshipThis investigation was supported by a grant from the NOVO Foundation and by Public Health Service grant AI 15286 from the National Institute of Allergy and Infectious Diseases.en
dc.description.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC217294/en
dc.format.extent7 pagesen
dc.genrejournal articlesen
dc.identifierdoi:10.13016/m2cqg7-bx8w
dc.identifier.citationJ M Zengel and L Lindahl, High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli, J Bacteriol. 1981 Jan; 145(1): 459–465, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC217294/en
dc.identifier.urihttp://hdl.handle.net/11603/14011
dc.language.isoenen
dc.publisherAmerican Society for Microbiology (ASM)en
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectamber mutationsen
dc.subjectEscherichia colien
dc.subjecttemperature-sensitiveen
dc.titleHigh-efficiency, temperature-sensitive suppression of amber mutations in Escherichia colien
dc.typeTexten

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