High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli
| dc.contributor.author | Zengel, J. M. | |
| dc.contributor.author | Lindahl, L. | |
| dc.date.accessioned | 2019-06-04T18:07:50Z | |
| dc.date.available | 2019-06-04T18:07:50Z | |
| dc.date.issued | 1981-01 | |
| dc.description.abstract | We have constructed a high-copy-number plasmid carrying an allele of the supD gene (supD43,74). The plasmid conferred temperature-sensitive suppression of amber mutations. Strains carrying the plasmid exhibited 50 to 60% suppression at 30 degrees C but little or no suppression at 42 degrees C. After a temperature shift from 30 to 42 degrees C the efficiency of suppression decreased gradually over a 60- to 90-min period before reaching the 42 degrees C steady-state level of suppression. | en_US |
| dc.description.sponsorship | This investigation was supported by a grant from the NOVO Foundation and by Public Health Service grant AI 15286 from the National Institute of Allergy and Infectious Diseases. | en_US |
| dc.description.uri | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC217294/ | en_US |
| dc.format.extent | 7 pages | en_US |
| dc.genre | journal articles | en_US |
| dc.identifier | doi:10.13016/m2cqg7-bx8w | |
| dc.identifier.citation | J M Zengel and L Lindahl, High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli, J Bacteriol. 1981 Jan; 145(1): 459–465, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC217294/ | en_US |
| dc.identifier.uri | http://hdl.handle.net/11603/14011 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | American Society for Microbiology (ASM) | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Biological Sciences Department Collection | |
| dc.relation.ispartof | UMBC Faculty Collection | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.subject | amber mutations | en_US |
| dc.subject | Escherichia coli | en_US |
| dc.subject | temperature-sensitive | en_US |
| dc.title | High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli | en_US |
| dc.type | Text | en_US |
Files
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 2.56 KB
- Format:
- Item-specific license agreed upon to submission
- Description: