A Novel Genus of Actinobacterial Tectiviridae

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dc.contributor.authorCaruso, Steven M.
dc.contributor.authordeCarvalho, Tagide N.
dc.contributor.authorHuynh, Anthony
dc.contributor.authorMorcos, George
dc.contributor.authorKuo, Nansen
dc.contributor.authorParsa, Shabnam
dc.contributor.authorErill, Ivan
dc.date.accessioned2020-01-31T16:07:04Z
dc.date.available2020-01-31T16:07:04Z
dc.date.issued2019-12-07
dc.description.abstractStreptomyces phages WheeHeim and Forthebois are two novel members of the Tectiviridaefamily. These phages were isolated on cultures of the plant pathogen Streptomyces scabiei, known for its worldwide economic impact on potato crops. Transmission electron microscopy showed viral particles with double-layered icosahedral capsids, and frequent instances of protruding nanotubes harboring a collar-like structure. Mass-spectrometry confirmed the presence of lipids in the virion, and serial purification of colonies from turbid plaques and immunity testing revealed that both phages are temperate. Streptomyces phages WheeHeim and Forthebois have linear dsDNA chromosomes (18,266 bp and 18,251 bp long, respectively) with the characteristic two-segment architecture of the Tectiviridae. Both genomes encode homologs of the canonical tectiviral proteins (major capsid protein, packaging ATPase and DNA polymerase), as well as PRD1-type virion-associated transglycosylase and membrane DNA delivery proteins. Comparative genomics and phylogenetic analyses firmly establish that these two phages, together with Rhodococcus phage Toil, form a new genus within the Tectiviridae, which we have tentatively named Deltatectivirus. The identification of a cohesive clade of Actinobacteria-infecting tectiviruses with conserved genome structure but with scant sequence similarity to members of other tectiviral genera confirms that the Tectiviridae are an ancient lineage infecting a broad range of bacterial hosts.en_US
dc.description.sponsorshipResearch reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Numbers TL4GM118989, UL1GM118988, and RL5GM118987. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. This work was also supported by the UMBC Department of Biological Sciences and the Howard Hughes Medical Institute SEA-PHAGES program.en_US
dc.description.urihttps://www.mdpi.com/1999-4915/11/12/1134en_US
dc.format.extent18 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2lel6-ewob
dc.identifier.citationCaruso, Steven M.; deCarvalho, Tagide N.; Huynh, Anthony; Morcos, George; Kuo, Nansen; Parsa, Shabnam; Erill, Ivan; A Novel Genus of Actinobacterial Tectiviridae; Viruses 2019, 11(12); https://www.mdpi.com/1999-4915/11/12/1134en_US
dc.identifier.urihttps://doi.org/10.3390/v11121134
dc.identifier.urihttp://hdl.handle.net/11603/17199
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution 4.0 International (CC BY 4.0)*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjecttectivirusen_US
dc.subjectactinobacteriaen_US
dc.subjectstreptomycesen_US
dc.subjectpathogenen_US
dc.subjectplanten_US
dc.subjectlipid membraneen_US
dc.subjectcapsid proteinen_US
dc.subjectpotato scaben_US
dc.titleA Novel Genus of Actinobacterial Tectiviridaeen_US
dc.typeTexten_US

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