Analysis of the SOS response of Vibrio and other bacteria with multiple chromosomes

Sanchez-Alberola, Neus; Campoy, Susana; Barbé, Jordi; Erill, Ivan

Analysis of the SOS response of Vibrio and other bacteria with multiple chromosomes

dc.contributor.author	Sanchez-Alberola, Neus
dc.contributor.author	Campoy, Susana
dc.contributor.author	Barbé, Jordi
dc.contributor.author	Erill, Ivan
dc.date.accessioned	2021-03-08T20:06:17Z
dc.date.available	2021-03-08T20:06:17Z
dc.date.issued	2012-02-03
dc.description.abstract	Background The SOS response is a well-known regulatory network present in most bacteria and aimed at addressing DNA damage. It has also been linked extensively to stress-induced mutagenesis, virulence and the emergence and dissemination of antibiotic resistance determinants. Recently, the SOS response has been shown to regulate the activity of integrases in the chromosomal superintegrons of the Vibrionaceae, which encompasses a wide range of pathogenic species harboring multiple chromosomes. Here we combine in silico and in vitro techniques to perform a comparative genomics analysis of the SOS regulon in the Vibrionaceae, and we extend the methodology to map this transcriptional network in other bacterial species harboring multiple chromosomes. Results Our analysis provides the first comprehensive description of the SOS response in a family (Vibrionaceae) that includes major human pathogens. It also identifies several previously unreported members of the SOS transcriptional network, including two proteins of unknown function. The analysis of the SOS response in other bacterial species with multiple chromosomes uncovers additional regulon members and reveals that there is a conserved core of SOS genes, and that specialized additions to this basic network take place in different phylogenetic groups. Our results also indicate that across all groups the main elements of the SOS response are always found in the large chromosome, whereas specialized additions are found in the smaller chromosomes and plasmids. Conclusions Our findings confirm that the SOS response of the Vibrionaceae is strongly linked with pathogenicity and dissemination of antibiotic resistance, and suggest that the characterization of the newly identified members of this regulon could provide key insights into the pathogenesis of Vibrio. The persistent location of key SOS genes in the large chromosome across several bacterial groups confirms that the SOS response plays an essential role in these organisms and sheds light into the mechanisms of evolution of global transcriptional networks involved in adaptability and rapid response to environmental changes, suggesting that small chromosomes may act as evolutionary test beds for the rewiring of transcriptional networks.	en_US
dc.description.sponsorship	This work was supported by the UMBC Office of Research through a Special Research Assistantship/Initiative Support (SRAIS) award, and by grants BFU2008-01078/BMC from the Ministerio de Ciencia e Innovación de España and 2009SGR-1106 from the Generalitat de Catalunya. NSA was supported by a fellowship from the Fundació Cellex. Writing of this paper was supported by a UMBC SFF award. The authors are indebted to Dr. Richard E. Wolf for kindly allowing NSA to perform part of the experimental work reported here in his lab, and to Dr. Lanyn P. Taliaferro for her assistance in setting up EMSA. The authors wish to thank the two anonymous reviewers for their insightful comments and Dr. Michael C. O'Neill for proofreading the final version of this manuscript.	en_US
dc.description.uri	https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-13-58	en_US
dc.format.extent	12 pages	en_US
dc.genre	journal articles	en_US
dc.identifier	doi:10.13016/m2imdu-swsi
dc.identifier.citation	Sanchez-Alberola, N., Campoy, S., Barbé, J. et al. Analysis of the SOS response of Vibrio and other bacteria with multiple chromosomes. BMC Genomics 13, 58 (2012). https://doi.org/10.1186/1471-2164-13-58	en_US
dc.identifier.uri	https://doi.org/10.1186/1471-2164-13-58
dc.identifier.uri	http://hdl.handle.net/11603/21103
dc.language.iso	en_US	en_US
dc.publisher	BMC	en_US
dc.relation.isAvailableAt	The University of Maryland, Baltimore County (UMBC)
dc.relation.ispartof	UMBC Biological Sciences Department Collection
dc.relation.ispartof	UMBC Faculty Collection
dc.rights	This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rights	Attribution 2.0 Generic	*
dc.rights.uri	https://creativecommons.org/licenses/by/2.0/	*
dc.title	Analysis of the SOS response of Vibrio and other bacteria with multiple chromosomes	en_US
dc.type	Text	en_US