TRPM5-Expressing Solitary Chemosensory Cells Respond to Odorous Irritants

Simple item page
dc.contributor.authorLin, Weihong
dc.contributor.authorOgura, Tatsuya
dc.contributor.authorMargolskee, Robert F.
dc.contributor.authorFinger, Thomas E.
dc.contributor.authorRestrepo, Diego
dc.date.accessioned2021-02-23T18:07:19Z
dc.date.available2021-02-23T18:07:19Z
dc.date.issued2008-03-01
dc.description.abstractInhaled airborne irritants elicit sensory responses in trigeminal nerves innervating the nasal epithelium, leading to protective reflexes. The sensory mechanisms involved in the detection of odorous irritants are poorly understood. We identified a large population of solitary chemosensory cells expressing the transient receptor potential channel M5 (TRPM5) using transgenic mice where the promoter of TRPM5 drives the expression of green fluorescent protein (GFP). Most of these solitary chemosensory cells lie in the anterior nasal cavity. These GFP-labeled solitary chemosensory cells exhibited immunoreactivity for synaptobrevin-2, a vesicle-associated membrane protein important for synaptic transmission. Concomitantly, we found trigeminal nerve fibers apposed closely to the solitary chemosensory cells, indicating potential transmission of sensory information to trigeminal fibers. In addition, stimulation of the nasal cavity with high concentrations (0.5–5 mM) of a variety of odorants elicited event-related potentials (ERPs) in areas rich in TRPM5-expressing solitary chemosensory cells. Furthermore, odorous chemicals and trigeminal stimuli induced changes in intracellular Ca2+ levels in isolated TRPM5-expressing solitary chemosensory cells in a concentration-dependent manner. Together, our data show that the TRPM5-expressing cells respond to a variety of chemicals at high exposure levels typical of irritants and are positioned in the nasal cavity appropriately to monitor inhaled air quality.en_US
dc.description.sponsorshipWe thank Dr. Wayne Silver for discussions. This work was supported by National Institutes of Health grants to W. Lin, T. Ogura, R. F. Margolskee, T. E. Finger, and D. Restrepoen_US
dc.description.urihttps://journals.physiology.org/doi/full/10.1152/jn.01195.2007en_US
dc.format.extent10 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2vxlv-xlev
dc.identifier.citationWeihong Lin, Tatsuya Ogura, Robert F. Margolskee, Thomas E. Finger, and Diego Restrepo, TRPM5-Expressing Solitary Chemosensory Cells Respond to Odorous Irritants, JNP, Volume 99 Issue 3 (2008) Pages 1451-1460, DOI: https://doi.org/10.1152/jn.01195.2007en_US
dc.identifier.urihttps://doi.org/10.1152/jn.01195.2007
dc.identifier.urihttp://hdl.handle.net/11603/21069
dc.language.isoen_USen_US
dc.publisherAmerican Physiological Society (APS)en_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectairborne irritantsen_US
dc.subjectsensory perceptionen_US
dc.subjectodorantsen_US
dc.subjectnervesen_US
dc.subjectchemosensory cellsen_US
dc.titleTRPM5-Expressing Solitary Chemosensory Cells Respond to Odorous Irritantsen_US
dc.typeTexten_US

Files

License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.56 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UMBC Biological Sciences Department
UMBC Faculty Collection