RNA-structural Mimicry in Escherichia coli Ribosomal Protein L4-dependent Regulation of the S10 Operon

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dc.contributor.authorStelzl, Ulrich
dc.contributor.authorZengel, Janice M.
dc.contributor.authorTovbina, Marina
dc.contributor.authorWalker, Marquis
dc.contributor.authorNierhaus, Knud H.
dc.contributor.authorLindahl, Lasse
dc.contributor.authorPatel, Dinshaw J.
dc.date.accessioned2019-06-19T18:00:48Z
dc.date.available2019-06-19T18:00:48Z
dc.date.issued2003-07-25
dc.description.abstractRibosomal protein L4 regulates the 11-gene S10 operon in Escherichia coli by acting, in concert with transcription factor NusA, to cause premature transcription termination at a Rho-independent termination site in the leader sequence. This process presumably involves L4 interaction with the leader mRNA. Here, we report direct, specific, and independent binding of ribosomal protein L4 to the S10 mRNA leader in vitro. Most of the binding energy is contributed by a small hairpin structure within the leader region, but a 64-nucleotide sequence is required for the bona fide interaction. Binding to the S10 leader mRNA is competed by the 23 S rRNA L4 binding site. Although the secondary structures of the mRNA and rRNA binding sites appear different, phosphorothioate footprinting of the L4-RNA complexes reveals close structural similarity in three dimensions. Mutational analysis of the mRNA binding site is compatible with the structural model. In vitro binding of L4 induces structural changes of the S10 leader RNA, providing a first clue for how protein L4 may provoke transcription termination.en_US
dc.description.sponsorshipThis work was supported by the National Institutes of Health Grant CA46778 (to D. J. P.) and NIGMS, National Institutes of General Medical Science Grant 54876 (to J. M. Z. and L. L.).en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692380/en_US
dc.format.extent23 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m22nhi-oox9
dc.identifier.citationUlrich Stelzl, et.al, RNA-structural Mimicry in Escherichia coli Ribosomal Protein L4-dependent Regulation of the S10 Operon, J Biol Chem. 2003 Jul 25; 278(30): 28237–28245. Published online 2003 May 8, DOI: 10.1074/jbc.M302651200en_US
dc.identifier.urihttps://dx.doi.org/10.1074%2Fjbc.M302651200
dc.identifier.urihttp://hdl.handle.net/11603/14271
dc.language.isoen_USen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectEscherichia colien_US
dc.subjectbona fide interactionen_US
dc.subjectRibosomal Protein L4en_US
dc.titleRNA-structural Mimicry in Escherichia coli Ribosomal Protein L4-dependent Regulation of the S10 Operonen_US
dc.typeTexten_US

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