A Theoretical Method to determine unstressed off-rate from multiple bond force spectroscopy
| dc.contributor.author | Eggleton, C.D. | |
| dc.contributor.author | Gupta, V.K. | |
| dc.date.accessioned | 2018-10-22T13:25:21Z | |
| dc.date.available | 2018-10-22T13:25:21Z | |
| dc.date.issued | 2012-06-15 | |
| dc.description.abstract | Using dynamic force spectroscopy to measure the kinetic off-rates of intermolecular bonds currently requires the isolation of single molecules. This requirement arises in part because no tractable analytic method for determining kinetic off-rates from the rupture of a large number of bonds under dynamic forces is currently available. We introduce a novel method for determining the unstressed off-rate from dynamic force spectroscopy experiments involving a large number of bonds. Using both the Bell and Dembo models we show that the unstressed off-rate calculated using the proposed method is in good agreement with the prescribed unstressed off-rate used in Monte-Carlo simulations of multiple bond dynamic force spectroscopy experiments given initial number of bonds (50–500) and loading rate 10³ – 10⁶ pN/s. | en_US |
| dc.description.sponsorship | The authors would like to acknowledge the financial support provided by the National Institute of Health Grant RO1 AI063366. We thank the Pittsburgh Supercomputing Center for computational resources under DAC allocation MCB090098 and computational resources from HPCF at UMBC supported by the U.S. National Science Foundation through the MRI program (grant no. CNS-0821258) and the SCREMS program (grant no. DMS-0821311). The authors gratefully acknowledge helpful communication with David F.J. Tees. | en_US |
| dc.description.uri | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348403/ | en_US |
| dc.format.extent | 23 pages | en_US |
| dc.genre | journal article pre-print | en_US |
| dc.identifier | doi:10.13016/M23J39493 | |
| dc.identifier.citation | V.K. Gupta ,C.D. Eggleton, A Theoretical Method to determine unstressed off-rate from multiple bond force spectroscopy, Colloids Surf B Biointerfaces. 2012 June 15; 95: 50–56. doi:10.1016/j.colsurfb.2012.02.010 | en_US |
| dc.identifier.uri | 10.1016/j.colsurfb.2012.02.010 | |
| dc.identifier.uri | http://hdl.handle.net/11603/11624 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Elsevier B.V | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Mechanical Engineering Department Collection | |
| dc.relation.ispartof | UMBC Faculty Collection | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
| dc.subject | kinetic off-rates | en_US |
| dc.subject | multiple bond force | en_US |
| dc.subject | spectroscopy | en_US |
| dc.subject | Monte-Carlo simulations | en_US |
| dc.subject | UMBC High Performance Computing Facility (HPCF) | en_US |
| dc.title | A Theoretical Method to determine unstressed off-rate from multiple bond force spectroscopy | en_US |
| dc.type | Text | en_US |