Identification of a functional core in the RNA component of RNase MRP of budding yeasts

dc.contributor.authorLi, Xing
dc.contributor.authorZaman, Sephorah
dc.contributor.authorLangdon, Yvette
dc.contributor.authorZengel, Janice M.
dc.contributor.authorLindahl, Lasse
dc.date.accessioned2019-06-19T18:10:41Z
dc.date.available2019-06-19T18:10:41Z
dc.date.issued2004-07-14
dc.description.abstractRNase MRP is an endonuclease participating in ribosomal RNA processing. It consists of one RNA and at least nine protein subunits. Using oligonucleotide-directed mutagenesis, we analyzed the functional role of five of the hairpins in the secondary structure of the RNA subunit of Saccharomyces cerevisiae RNase MRP. Deletion of an entire hairpin was either lethal or resulted in very poor growth. However, peripheral portions constituting up to 70% of a hairpin could be deleted without effects on cell growth rate or processing of rRNA. To determine whether these hairpins perform redundant functions, we analyzed mutants combining four or five benign hairpin deletions. Simultaneous removal of four of these hairpin segments had no detectable effect. Removing five created a temperature- and cold-sensitive enzyme, but these deficiencies could be partially overcome by a mutation in one of the RNase MRP protein subunits, or by increasing the copy number of several of the protein subunit genes. These observations suggest that the peripheral elements of the RNA hairpins contain no structures or sequences required for substrate recognition, catalysis or binding of protein subunits. Thus, the functionally essential elements of the RNase MRP RNA appear to be concentrated in the core of the subunit.en_US
dc.description.sponsorshipThis work was supported by Grant MCB 0077949 from the National Science Foundation to L.L. and J.M.Z.en_US
dc.description.urihttps://watermark.silverchair.com/gkh689.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAkcwggJDBgkqhkiG9w0BBwagggI0MIICMAIBADCCAikGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMCNcDJCxbwiY5zwvOAgEQgIIB-sVCb-XIZHcqV2_RTvpMgYK55pTphhcHv50skaEBb64iSzJETr-vTb7hhz6YsoVVPBNWKGZIcBXA_yI-e9h_Xvmvt0EoSTMQF70YJ24ryv6xgMAV0IMgp6IrXVr2PbelBTxOM-ONv-8wb0Vu-Vk_vdlbqYm84yLcEUo_EXbeAJSZaCjMksTNrSQjviWVzfj1D6TiKISzcOEqcE8t7MZ9Ub0gZXwdCBgZU_ojBK8PEsDEZkXjXCSz_VdLeOP5OzaOsBV77PuYOE_IJKdbQE8DhMFAwsYdl6v-GP9Lgo99YqxlaMnWnEol3XkG0904CST2yczlDhIfvlol8Z6BZZGKl1ecgVBDHk2EcCHevjJg27TpT56wYElKUZhStLrl-ziGG_MPuhRClDHUSLKw3y9nwvF3eOSpuzn0xjB8isFI_8qgtjExUwhnPjiPW1WVFO1h2Gx4jnnpL5mIHuyVNp0kzbvGFVx0jsXTS2QBXYK6lsTgg5lJokltqvFcHALBJsf5MA6Niknjqq_XVhoUuR11u4uWTsZLFMzVpJfp885IStEBDUSkX25xFnYqWINvi9Wcwd7h-xKTBU_upSljb2EXQ0DVuMtInreykicwa3qA_7UXYZxBg1-eNOChEDntyR_sN-53D0HshlAEKnDvppCjNurlHeojZrpVYJ_Xen_US
dc.format.extent9 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2jyla-ge3t
dc.identifier.citationXing Li, et.al, Identification of a functional core in the RNA component of RNase MRP of budding yeasts, Nucleic Acids Res. 2004; 32(12): 3703–3711, 2004.. DOI: 10.1093/nar/gkh689en_US
dc.identifier.urihttps://dx.doi.org/10.1093%2Fnar%2Fgkh689
dc.identifier.urihttp://hdl.handle.net/11603/14273
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectribosomal RNAen_US
dc.subjectendonucleaseen_US
dc.titleIdentification of a functional core in the RNA component of RNase MRP of budding yeastsen_US
dc.typeTexten_US

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