Recombinant Newcastle Disease Virus (NDV) Expressing Sigma C Protein of Avian Reovirus (ARV) Protects against Both ARV and NDV in Chickens
| dc.contributor.author | Saikia, Deep Prakash | |
| dc.contributor.author | Yadav, Kalpana | |
| dc.contributor.author | Pathak, Dinesh C. | |
| dc.contributor.author | Ramamurthy, Narayan | |
| dc.contributor.author | D’Silva, Ajai Lawrence | |
| dc.contributor.author | Marriappan, Asok Kumar | |
| dc.contributor.author | Ramakrishnan, Saravanan | |
| dc.contributor.author | Vakharia, Vikram N. | |
| dc.contributor.author | Chellappa, Madhan Mohan | |
| dc.contributor.author | Dey, Sohini | |
| dc.date.accessioned | 2019-10-18T14:54:13Z | |
| dc.date.available | 2019-10-18T14:54:13Z | |
| dc.date.issued | 2019-09-10 | |
| dc.description.abstract | Newcastle disease (ND) and avian reovirus (ARV) infections are a serious threat to the poultry industry, which causes heavy economic losses. The mesogenic NDV strain R2B is commonly used as a booster vaccine in many Asian countries to control the disease. In this seminal work, a recombinant NDV strain R2B expressing the sigma C (σC) gene of ARV (rNDV-R2B-σC) was generated by reverse genetics, characterized in vitro and tested as a bivalent vaccine candidate in chickens. The recombinant rNDV-R2B-σC virus was attenuated as compared to the parent rNDV-R2B virus as revealed by standard pathogenicity assays. The generated vaccine candidate, rNDV-R2B-σC, could induce both humoral and cell mediated immune responses in birds and gave complete protection against virulent NDV and ARV challenges. Post-challenge virus shedding analysis revealed a drastic reduction in NDV shed, as compared to unvaccinated birds | en |
| dc.description.sponsorship | The research work was supported by grants from the Department of Biotechnology, Government of India (BT/PR15373/AAQ/57/116/2011 and BT/PR21576/ADV/90/249/2016) and National Agricultural Science Fund, ICAR (NFBSFARA/BS-3010). | en |
| dc.description.uri | https://www.mdpi.com/2076-0817/8/3/145 | en |
| dc.format.extent | 21 pages | en |
| dc.genre | journal articles | en |
| dc.identifier | doi:10.13016/m2toyz-kto8 | |
| dc.identifier.citation | Saikia, D.P.; Yadav, K.; Pathak, D.C.; Ramamurthy, N.; D’Silva, A.L.; Marriappan, A.K.; Ramakrishnan, S.; Vakharia, V.N.; Chellappa, M.M.; Dey, S. Recombinant Newcastle Disease Virus (NDV) Expressing Sigma C Protein of Avian Reovirus (ARV) Protects against Both ARV and NDV in Chickens. Pathogens 2019, 8, 145. | en |
| dc.identifier.uri | https://doi.org/10.3390/pathogens8030145 | |
| dc.identifier.uri | http://hdl.handle.net/11603/15913 | |
| dc.language.iso | en | en |
| dc.publisher | MDPI | en |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Department of Marine Biotechnology | |
| dc.relation.ispartof | UMBC Faculty Collection | |
| dc.rights | Attribution 4.0 International (CC BY 4.0) | * |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject | newcastle disease virus | en |
| dc.subject | avian reovirus-σC protein | en |
| dc.subject | bivalent vaccine candidate | en |
| dc.subject | humoral and cell mediated immune responses | en |
| dc.subject | protection efficacy | en |
| dc.title | Recombinant Newcastle Disease Virus (NDV) Expressing Sigma C Protein of Avian Reovirus (ARV) Protects against Both ARV and NDV in Chickens | en |
| dc.type | Text | en |