Ultra-Fast and Sensitive Detection of Non-Typhoidal Salmonella Using Microwave-Accelerated Metal-Enhanced Fluorescence (“MAMEF”)

dc.contributor.authorTennant, Sharon M.
dc.contributor.authorZhang, Yongxia
dc.contributor.authorGalen, James E.
dc.contributor.authorGeddes, Chris
dc.contributor.authorLevine, Myron M.
dc.date.accessioned2024-10-01T18:05:33Z
dc.date.available2024-10-01T18:05:33Z
dc.date.issued2011-04-08
dc.description.abstractCertain serovars of Salmonella enterica subsp. enterica cause invasive disease (e.g., enteric fever, bacteremia, septicemia, meningitis, etc.) in humans and constitute a global public health problem. A rapid, sensitive diagnostic test is needed to allow prompt initiation of therapy in individual patients and for measuring disease burden at the population level. An innovative and promising new rapid diagnostic technique is microwave-accelerated metal-enhanced fluorescence (MAMEF). We have adapted this assay platform to detect the chromosomal oriC locus common to all Salmonella enterica subsp. enterica serovars. We have shown efficient lysis of biologically relevant concentrations of Salmonella spp. suspended in bacteriological media using microwave-induced lysis. Following lysis and DNA release, as little as 1 CFU of Salmonella in 1 ml of medium can be detected in <30 seconds. Furthermore the assay is sensitive and specific: it can detect oriC from Salmonella serovars Typhi, Paratyphi A, Paratyphi B, Paratyphi C, Typhimurium, Enteritidis and Choleraesuis but does not detect Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae or Acinetobacter baumanii. We have also performed preliminary experiments using a synthetic Salmonella oriC oligonucleotide suspended in whole human blood and observed rapid detection when the sample was diluted 1:1 with PBS. These pre-clinical data encourage progress to the next step to detect Salmonella in blood (and other ordinarily sterile, clinically relevant body fluids).
dc.description.sponsorshipThis work was funded by the Middle Atlantic RCE Program, National Institute of Allergy and Infectious Diseases/National Institutes of Health (NIAID/NIH) 2 U54 AI057168-06. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.description.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0018700
dc.format.extent8 pages
dc.genrejournal articles
dc.identifierdoi:10.13016/m2gqh6-msnm
dc.identifier.citationTennant, Sharon M., Yongxia Zhang, James E. Galen, Chris D. Geddes, and Myron M. Levine. “Ultra-Fast and Sensitive Detection of Non-Typhoidal Salmonella Using Microwave-Accelerated Metal-Enhanced Fluorescence (‘MAMEF’).” PLOS ONE 6, no. 4 (April 8, 2011): e18700. https://doi.org/10.1371/journal.pone.0018700.
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0018700
dc.identifier.urihttp://hdl.handle.net/11603/36587
dc.language.isoen_US
dc.publisherPLOS
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Institute of Fluorescence (IoF)
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Chemistry & Biochemistry Department
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectSalmonella enterica
dc.subjectSalmonella
dc.subjectSalmonella typhimurium
dc.subjectDNA isolation
dc.subjectSalmonella typhi
dc.subjectLysis (medicine)
dc.subjectMicrowave radiation
dc.subjectBlood
dc.titleUltra-Fast and Sensitive Detection of Non-Typhoidal Salmonella Using Microwave-Accelerated Metal-Enhanced Fluorescence (“MAMEF”)
dc.typeText
dcterms.creatorhttps://orcid.org/0000-0002-9110-6374

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