Assessment of Elasticity and Topography of Aspergillus nidulans Spores via Atomic Force Microscopy

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dc.contributor.authorZhao, Liming
dc.contributor.authorSchaefer, David
dc.contributor.authorMarten, Mark R.
dc.date.accessioned2019-02-01T15:28:55Z
dc.date.available2019-02-01T15:28:55Z
dc.date.issued2004-09-27
dc.description.abstractPrevious studies have described both surface morphology and adhesive properties of fungal spores, but little information is currently available on their mechanical properties. In this study, atomic force microscopy (AFM) was used to investigate both surface topography and micromechanical properties of Aspergillus nidulans spores. To assess the influence of proteins covering the spore surface, wild-type spores were compared with spores from isogenic rodA⁺ and rodA⁻ strains. Tapping-mode AFM images of wild-type and rodA⁺ spores in air showed characteristic “rodlet” protein structures covering a granular spore surface. In comparison, rodA spores were rodlet free but showed a granular surface structure similar to that of the wild-type and rodA⁺ spores. Rodlets were removed from rodA⁺ spores by sonication, uncovering the underlying granular layer. Both rodlet-covered and rodlet-free spores were subjected to nanoindentation measurements, conducted in air, which showed the stiffnesses to be 110 ± 10, 120 ± 10, and 300 ± 20 N/m and the elastic moduli to be 6.6 ± 0.4, 7.0 ± 0.7, and 22 ± 2 GPa for wild-type, rodA⁺ and rodA⁻ spores, respectively. These results imply the rodlet layer is significantly softer than the underlying portion of the cell wall.en_US
dc.description.sponsorshipThis work was supported by grants BES-9876012 and BES-9906586 from the National Science Foundation and by Novozymes North America, Inc.en_US
dc.description.urihttps://aem.asm.org/content/71/2/955en_US
dc.format.extent6 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2zzkr-x2xf
dc.identifier.citationLiming Zhao, David Schaefer, Mark R. Marten, Assessment of Elasticity and Topography of Aspergillus nidulans Spores via Atomic Force Microscopy, Applied and Environmental Microbiology, p. 955–960, 2005 , DOI: 10.1128/AEM.71.2.955-960.2005en_US
dc.identifier.uri10.1128/AEM.71.2.955-960.2005
dc.identifier.urihttp://hdl.handle.net/11603/12682
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectelasticityen_US
dc.subjecttopographyen_US
dc.subjectaspergillus nidulansen_US
dc.subjectsporesen_US
dc.subjectatomic force microscopeen_US
dc.titleAssessment of Elasticity and Topography of Aspergillus nidulans Spores via Atomic Force Microscopyen_US
dc.typeTexten_US

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