Fluorescence of crayfish metarhodopsin studied in single rhabdoms

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dc.contributor.authorCronin, T.W.
dc.contributor.authorGoldsmith, T.H.
dc.date.accessioned2019-05-24T14:18:14Z
dc.date.available2019-05-24T14:18:14Z
dc.date.issued1981-09
dc.description.abstractIsolated photoreceptor organelles (rhabdoms) from eyes of crayfish (Procambarus. Orconectes) were examined on a microscope system designed for quantitative measurements of fluorescent. Although fully dark-adapted rhabdoms are nonfluorescent or very weakly fluorescent, an increasing emission appears on exposure to light. Over the 30-fold range of intensities studied, the rate of the appearance of this fluorescence is identical to the rate of formation of metarhodopsin from rhodopsin. Furthermore, the excitation spectra for the observed emission are similar to the absorption spectra of crayfish metarhodopsin at both neutral and acid pH. Finally, the amount of fluorescence observed in rhabdoms previously irradiated with selected wavelengths of light is proportional to the amount of metarhodopsin present in the photosteady state established by the prior irradiation. The emission therefore originates from crayfish metarhodopsin. Fluorescence emission peaks at 670 nm at neutral pH. The quantum efficiency is 1.6 +/- 0.4 X 10(-3). Although emission from other rhodopsin photoproducts has previously been noted, this is the first description of fluorescence from the metarhodopsin chromophore site.en_US
dc.description.sponsorshipThis work was supported by National Institutes of Health grant EY00222 to Yale University.en_US
dc.description.urihttps://www.sciencedirect.com/science/article/pii/S0006349581848187?via%3Dihub#!en_US
dc.format.extent12 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2xvty-iguu
dc.identifier.citationT.W.Cronin, T.H.Goldsmith, Fluorescence of crayfish metarhodopsin studied in single rhabdoms, Biophysical Journal Volume 35, Issue 3, September 1981, Pages 653-664, https://doi.org/10.1016/S0006-3495(81)84818-7en_US
dc.identifier.urihttps://doi.org/10.1016/S0006-3495(81)84818-7
dc.identifier.urihttp://hdl.handle.net/11603/13936
dc.language.isoen_USen_US
dc.publisherElsevier Inc.en_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectcrayfishen_US
dc.subjectIsolated photoreceptor organelles (rhabdoms)en_US
dc.subjectmetarhodopsinen_US
dc.subjectchromophore siteen_US
dc.titleFluorescence of crayfish metarhodopsin studied in single rhabdomsen_US
dc.typeTexten_US

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