Genetic and bioprocess engineering to improve squalene production in Yarrowia lipolytica
| dc.contributor.author | Liu, Huan | |
| dc.contributor.author | Wang, Fang | |
| dc.contributor.author | Deng, Li | |
| dc.contributor.author | Xu, Peng | |
| dc.date.accessioned | 2020-08-17T16:18:57Z | |
| dc.date.available | 2020-08-17T16:18:57Z | |
| dc.date.issued | 2020-08-10 | |
| dc.description.abstract | Squalene is the precursor for triterpene-based natural products and steroids-based drugs. It has been widely used as pharmaceutical intermediates and personal care products. The aim of this work is to test the feasibility of engineering Yarrowia lipolytica as a potential host for squalene production. The bottleneck of the pathway was removed by overexpressing native HMG-CoA (3-hydroxy-3-methylglutaryl-CoA) reductase. With the recycling of NADPH from the mannitol cycle, the engineered strain produced about 180.3 mg/L and 188.2 mg/L squalene from glucose or acetate minimal media. By optimizing the C/N ratio, controlling the media pH and mitigating acetyl-CoA flux competition from lipogenesis, the engineered strain produced 502.7 mg/L squalene, a 28-fold increase over the parental strain (17.2 mg/L). This work may serve as a baseline to harness Y. lipolytica as an oleaginous cell factory for sustainable production of squalene or terpenoids-based chemicals and natural products. | en_US |
| dc.description.sponsorship | This work is supported by Bill & Melinda Gates Foundation (grantnumber OPP1188443) and National Science Foundation (CBET-1805139). HL would like to thank the China Scholarship Council forfunding support. | en_US |
| dc.description.uri | https://www.sciencedirect.com/science/article/pii/S0960852420312633?via%3Dihub | en_US |
| dc.format.extent | 8 pages | en_US |
| dc.genre | journal articles | en_US |
| dc.identifier | doi:10.13016/m2x8wy-swuq | |
| dc.identifier.citation | Huan Liu, Fang Wang, Li Deng and Peng Xu, Genetic and bioprocess engineering to improve squalene production in Yarrowia lipolytica, Bioresource Technology Volume 317, 123991 (2020), https://doi.org/10.1016/j.biortech.2020.123991 | en_US |
| dc.identifier.uri | https://doi.org/10.1016/j.biortech.2020.123991 | |
| dc.identifier.uri | http://hdl.handle.net/11603/19438 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Elsevier | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Chemical, Biochemical & Environmental Engineering Department Collection | |
| dc.relation.ispartof | UMBC Faculty Collection | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | Attribution 4.0 International | * |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
| dc.title | Genetic and bioprocess engineering to improve squalene production in Yarrowia lipolytica | en_US |
| dc.type | Text | en_US |