Acetylcholine Increases Intracellular Ca2+ in Taste Cells Via Activation of Muscarinic Receptors

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dc.contributor.authorOgura, Tatsuya
dc.date.accessioned2021-02-23T18:57:06Z
dc.date.available2021-02-23T18:57:06Z
dc.date.issued2002-06-01
dc.descriptionUMBC Weihong Lin Laben_US
dc.description.abstractPrevious studies suggest that acetylcholine (ACh) is a transmitter released from taste cells as well as a transmitter in cholinergic efferent neurons innervating taste buds. However, the physiological effects on taste cells have not been established. I examined effects of ACh on taste-receptor cells by monitoring [Ca2+]i. ACh increased [Ca2+]i in both rat and mudpuppy taste cells. Atropine blocked the ACh response, butd-tubocurarine did not. U73122, a phospholipase C inhibitor, and thapsigargin, a Ca2+-ATPase inhibitor that depletes intracellular Ca2+stores, blocked the ACh response. These results suggest that ACh binds to M1/M3/M5-like subtypes of muscarinic ACh receptors, causing an increase in inositol 1,4,5-trisphosphate and subsequent release of Ca2+ from the intracellular stores. A long incubation with ACh induced a transient response followed by a sustained phase of [Ca2+]i increase. In Ca2+-free solution, the sustained phases disappeared, suggesting that Ca2+ influx is involved in the sustained phase. Depletion of Ca2+ stores by thapsigargin alone induced Ca2+ influx. These findings suggest that Ca2+ store-operated channels may be present in taste cells and that they may participate in the sustained phase of [Ca2+]i increase. Immunocytochemical experiments indicated that the M1 subtype of muscarinic receptors is present in both rat and mudpuppy taste cells.en_US
dc.description.sponsorshipThe author thanks Dr. Sue C. Kinnamon at Colorado State University for financial support and helpful discussions as well as Dr. Weihong Lin for help with immunohystochemistry and Dr. Thomas E. Finger for help with confocal microscopy. This work was supported by National Institute of Deafness and Other Communication Disorders Grants DC-00766 and DC-00244 to Dr. Sue C. Kinnamonen_US
dc.description.urihttps://journals.physiology.org/doi/full/10.1152/jn.2002.87.6.2643en_US
dc.format.extent7 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2kdmk-n1oq
dc.identifier.citationTatsuya Ogura, Acetylcholine Increases Intracellular Ca2+ in Taste Cells Via Activation of Muscarinic Receptors, J Neurophysiol 87: 2643–2649, 2002; 10.1152/jn.00610.2001en_US
dc.identifier.urihttps://doi.org/10.1152/jn.2002.87.6.2643
dc.identifier.urihttp://hdl.handle.net/11603/21073
dc.language.isoen_USen_US
dc.publisherAmerican Physiological Society (APS)en_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjecttaste cellsen_US
dc.subjectmuscarinic receptorsen_US
dc.subjectneurotransmittersen_US
dc.subjectnerve fibersen_US
dc.titleAcetylcholine Increases Intracellular Ca2+ in Taste Cells Via Activation of Muscarinic Receptorsen_US
dc.typeTexten_US

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