Ultra-fast pg/ml anthrax toxin (protective antigen) detection assay based on microwave-accelerated metal-enhanced fluorescence

dc.contributor.authorDragan, Anatoliy
dc.contributor.authorAlbrecht, Mark T.
dc.contributor.authorPavlovic, Radmila
dc.contributor.authorKeane-Myers, Andrea M.
dc.contributor.authorGeddes, Chris
dc.date.accessioned2024-10-01T18:05:31Z
dc.date.available2024-10-01T18:05:31Z
dc.date.issued2012-06-01
dc.description.abstractRapid presymptomatic diagnosis of Bacillus anthracis at early stages of infection plays a crucial role in prompt medical intervention to prevent rapid disease progression and accumulation of lethal levels of toxin. To detect low levels of the anthrax protective antigen (PA) exotoxin in biological fluids, we have developed a metal-enhanced fluorescence (MEF)–PA assay using a combination of the MEF effect and microwave-accelerated PA protein surface absorption. The assay is based on a modified version of our “rapid catch and signal” (RCS) technology previously designed for the ultra-fast and sensitive analysis of genomic DNA sequences. Technologically, the proposed MEF–PA assay uses standard 96-well plastic plates modified with silver island films (SiFs) grown within the wells. It is shown that the fluorescent probe, covalently attached to the secondary antibody, plays a crucial role of indicating complex formation (i.e., shows a strong MEF response to the recognition event). Microwave irradiation rapidly accelerates PA deposition onto the surface (“rapid catch”), significantly speeding up the MEF–PA assay and resulting in a total assay run time of less than 40min with an analytical sensitivity of less than 1pg/ml PA.
dc.description.sponsorshipThe authors thank the Biological Defense Research Directorate, Naval Medical Research Center, for financial support (work unit number 00000123002) and are grateful for the financial support of the Institute of Fluorescence (IoF) at the University of Maryland, Baltimore County. The authors also acknowledge the support and discussions of Les Baillie (Cardiff University, UK). The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, or US government.
dc.description.urihttps://www.sciencedirect.com/science/article/pii/S0003269712001571
dc.format.extent8 pages
dc.genrejournal articles
dc.identifierdoi:10.13016/m2sky9-gzeq
dc.identifier.citationDragan, Anatoliy I., Mark T. Albrecht, Radmila Pavlovic, Andrea M. Keane-Myers, and Chris D. Geddes. “Ultra-Fast Pg/Ml Anthrax Toxin (Protective Antigen) Detection Assay Based on Microwave-Accelerated Metal-Enhanced Fluorescence.” Analytical Biochemistry 425, no. 1 (June 1, 2012): 54–61. https://doi.org/10.1016/j.ab.2012.02.040.
dc.identifier.urihttps://doi.org/10.1016/j.ab.2012.02.040
dc.identifier.urihttp://hdl.handle.net/11603/36583
dc.language.isoen_US
dc.publisherElsevier
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Institute of Fluorescence (IoF)
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Chemistry & Biochemistry Department
dc.rightsThis work was written as part of one of the author's official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.
dc.rightsPublic Domain
dc.rights.urihttps://creativecommons.org/publicdomain/mark/1.0/
dc.subjectSurface-enhanced fluorescence
dc.subjectProtective antigen (PA)
dc.subjectPoint-of care diagnostics
dc.subjectPlasmon-controlled fluorescence
dc.subjectPlasmon-enhanced fluorescence
dc.subjectBio-warfare agent detection
dc.subjectMetal-enhanced fluorescence
dc.subjectAnthrax
dc.subjectPathogen detection
dc.titleUltra-fast pg/ml anthrax toxin (protective antigen) detection assay based on microwave-accelerated metal-enhanced fluorescence
dc.typeText
dcterms.creatorhttps://orcid.org/0000-0002-1679-8307
dcterms.creatorhttps://orcid.org/0000-0002-9110-6374

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