Top-down Proteomic Characterization of Truncated Proteoforms

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dc.contributor.authorChen, Dapeng
dc.contributor.authorGeis-Asteggiante, Lucia
dc.contributor.authorGomes, Fabio
dc.contributor.authorOstrand-Rosenberg, Suzanne
dc.contributor.authorFenselau, Catherine
dc.date.accessioned2019-11-14T18:01:01Z
dc.date.available2019-11-14T18:01:01Z
dc.date.issued2019-09-10
dc.description.abstractA top-down proteomic strategy with semi-automated analysis of data sets has proven successful for global identification of truncated proteins without the use of chemical derivatization, enzymatic manipulation, immunoprecipitation or other enrichment. This approach provides the reliable identification of internal polypeptides formed from precursor gene products by proteolytic cleavage of both the N- and C-termini, as well as truncated proteoforms that retain one or the other termini. The strategy has been evaluated by application to the immunosuppressive extracellular vesicles released by myeloid-derived suppressor cells. More than 1000 truncated proteoforms have been identified, from which binding motifs are derived to allow characterization of the putative proteases responsible for truncation.en_US
dc.description.sponsorshipSupport is acknowledged by the following grants from the US National Institutes of Health: GM21248, OD019938, CA84232, CA115880. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.en_US
dc.description.urihttps://pubs.acs.org/doi/10.1021/acs.jproteome.9b00487en_US
dc.format.extent15 pagesen_US
dc.genrejournal articles postprintsen_US
dc.identifierdoi:10.13016/m2mfus-phkf
dc.identifier.citationChen, Dapeng; Geis-Asteggiante, Lucia; Gomes, Fabio; Ostrand-Rosenberg, Suzanne; Fenselau, Catherine; Top-down Proteomic Characterization of Truncated Proteoforms; Journal of Proteome Research 18,11 (2019); https://doi.org/10.1021/acs.jproteome.9b00487en_US
dc.identifier.urihttps://doi.org/10.1021/acs.jproteome.9b00487
dc.identifier.urihttp://hdl.handle.net/11603/16313
dc.language.isoen_USen_US
dc.publisherACS Publicationsen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemistry & Biochemistry Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAccess to this item will begin on 2020-09-23
dc.rightsThis document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Journal of Proteome Research, copyright ©American Chemical Society after peer review. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.jproteome.9b00487.
dc.subjecttruncated proteoformsen_US
dc.subjecttop-down mass spectrometryen_US
dc.subjectcleavage motifsen_US
dc.subjectprotease databaseen_US
dc.subjectextracellular vesiclesen_US
dc.subjectmyeloid-derived suppressor cellsen_US
dc.titleTop-down Proteomic Characterization of Truncated Proteoformsen_US
dc.typeTexten_US

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