Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p

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dc.contributor.authorCheeseman, Iain M
dc.contributor.authorAnderson, Scott
dc.contributor.authorJwa, Miri
dc.contributor.authorGreen, Erin
dc.contributor.authorKang, Jung seog
dc.contributor.author3rd, John R Yates
dc.contributor.authorChan, Clarence S M
dc.contributor.authorDrubin, David G
dc.contributor.authorBarnes, Georjana
dc.date.accessioned2023-01-25T15:11:06Z
dc.date.available2023-01-25T15:11:06Z
dc.date.issued2002-10-18
dc.description.abstractThe Aurora kinase Ipl1p plays a crucial role in regulating kinetochore-microtubule attachments in budding yeast, but the underlying basis for this regulation is not known. To identify Ipl1p targets, we first purified 28 kinetochore proteins from yeast protein extracts. These studies identified five previously uncharacterized kinetochore proteins and defined two additional kinetochore subcomplexes. We then used mass spectrometry to identify 18 phosphorylation sites in 7 of these 28 proteins. Ten of these phosphorylation sites are targeted directly by Ipl1p, allowing us to identify a consensus phosphorylation site for an Aurora kinase. Our systematic mutational analysis of the Ipl1p phosphorylation sites demonstrated that the essential microtubule binding protein Dam1p is a key Ipl1p target for regulating kinetochore-microtubule attachments in vivo.en_US
dc.description.sponsorshipThe authors thank A. Desai, R. Heald, and S. Gadde for critical reading of the manuscript; J. Thorner for useful discussions; and M. Stark for strains. This work was supported by grants from the NIGMS to G.B. (GM-47842) and C.S.M.C. (GM-45185), a grant to S. Fields and the Yeast Research Consortium from the National Center for Research Resources of the NIH (Comprehensive Biology: Exploiting the Yeast Genome; PHS # P41 RR11823), and an NSF Graduate Research Fellowship to I.M.C.en_US
dc.description.urihttps://www.cell.com/cell/fulltext/S0092-8674(02)00973-X?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS009286740200973X%3Fshowall%3Dtrueen_US
dc.format.extent10 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2kozr-pfjp
dc.identifier.citationCheeseman, Iain M et al. “Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p.” Cell vol. 111,2 (2002): 163-72. doi:10.1016/s0092-8674(02)00973-xen_US
dc.identifier.urihttps://doi.org/10.1016/S0092-8674(02)00973-X
dc.identifier.urihttp://hdl.handle.net/11603/26705
dc.language.isoen_USen_US
dc.publisherCell Pressen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.en_US
dc.subjectkinetochore-microtubuleen_US
dc.subjectIpl1pen_US
dc.titlePhospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1pen_US
dc.typeTexten_US
dcterms.creatorhttps://orcid.org/0000-0003-3923-6726en_US

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