Blood Serum Affects Polysaccharide Production and Surface Protein Expression in S. Aureus

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dc.contributor.authorIslam, Nazrul
dc.contributor.authorHossain, Khwaja G.
dc.contributor.authorRoss, Julia M.
dc.contributor.authorMarten, Mark R.
dc.date.accessioned2019-02-11T15:45:54Z
dc.date.available2019-02-11T15:45:54Z
dc.date.issued2017-03-03
dc.description.abstractBackground: S. aureus biofilm serves a major role in pathogenesis. Two of the major components of bacterial biofilm are Polysaccharides intercellular adhesions (PIA) and surface proteins. It is not known how PIA and surface proteins expressions are affected in presence of blood serum. Analyses of surface proteins expressions will provide more effective biomarker discovery that might lead to development of antimicrobial therapeutics to meet the challenges of biofilm-related infections. Method: Secondary cultures of S. aureus Philips, a biofilm-forming bacterium, were generated by inoculating 1 ml of overnight culture into 50 ml of TSB. Bacteria were cultured at several concentrations of blood serum and found that 12.5% supplemented blood serum provide s similar growth curve as normal TSB (100%). One and 2 D SASPAGE were used to separate proteins and the differentially expressed proteins were identified by nano-LC/MS. Results: Polysaccharide intercellular adhesions production was significantly increased due to the addition of blood serum in the media. We also identified two serum proteins, apolipoprotein and globulin (Fc and Fab), that remained attached with the membrane fraction of bacterial proteins. Conclusion: These results have strongly demonstrated that blood serum influences the exopolysaccharide expression in S. aureus.en_US
dc.description.sponsorshipThis research was supported by Grant no. RO1AI059369 from the NIH and also an Institutional Development Award (IDeA) under NIH grant no. P20GM103442.en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pubmed/29863159en_US
dc.genrejournal aritclesen_US
dc.identifierdoi:10.13016/m2r8ce-7bly
dc.identifier.citationIslam N, Hossain KG, Ross JM, Marten MR, Blood Serum Affects Polysaccharide Production and Surface Protein Expression in S. Aureus, Adv Biotechnol Microbiol. 2017;2(3). pii: 555589. doi: 10.19080/ARGH.2017.02.555589en_US
dc.identifier.uri10.19080/ARGH.2017.02.555589
dc.identifier.urihttp://hdl.handle.net/11603/12758
dc.language.isoen_USen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectbiofilmen_US
dc.subjectproteomeen_US
dc.subjectserumen_US
dc.subjectstaphylococcus aureusen_US
dc.titleBlood Serum Affects Polysaccharide Production and Surface Protein Expression in S. Aureusen_US
dc.typeTexten_US

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