Photosensitivity spectrum of crayfish rhodopsin measured using fluorescence of metarhodopsin

Simple item page
dc.contributor.authorCronin, T. W.
dc.contributor.authorGoldsmith, T. H.
dc.date.accessioned2019-05-24T14:31:03Z
dc.date.available2019-05-24T14:31:03Z
dc.date.issued1982-02-01
dc.description.abstractDiscrepancies exist among spectral measurements of sensitivity of crayfish photoreceptors, their absorption in situ, and the number and absorption spectra of crayfish photopigments that are extracted by digitonin solutions. We have determined the photosensitivity spectrum of crayfish rhodopsin in isolated rhabdoms using long wavelength fluorescence emission from crayfish metarhodopsin as an intrinsic probe. There is no measurable metarhodopsin in the dark-adapted receptor, so changes in the emission level are directly proportional to metarhodopsin concentration. We therefore used changes in metarhodopsin fluorescence to construct relaxation and saturation ("photoequilibrium") spectra, from which the photosensitivity spectrum of crayfish rhodopsin was calculated. This spectrum peaks at or approximately 530 nm and closely resembles the previously measured difference spectrum for total bleaches of dark-adapted rhabdoms. Measurements of the kinetics of changes in rhabdom fluorescence and in transmittance at 580 nm were compared with predictions derived from several model systems containing one or two photopigments. The comparison shows that only a single rhodopsin and its metarhodopsin are present in the main rhabdom of crayfish, and that other explanations must be sought for the multiple pigments seen in digitonin solution. The same analysis shows that there is no detectable formation of isorhodopsin in the rhabdom.en_US
dc.description.sponsorshipThis work was supported by NIH grant EY00222 to Yale Universit.en_US
dc.description.urihttp://jgp.rupress.org/content/79/2/313en_US
dc.format.extent20 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2mr84-bd2x
dc.identifier.citationT. W. Cronin, T. H. Goldsmith, Photosensitivity spectrum of crayfish rhodopsin measured using fluorescence of metarhodopsin, JGP Home , 1982 Archive, 1 February, 79 (2): 313, https://doi.org/10.1085/jgp.79.2.313en_US
dc.identifier.urihttps://doi.org/10.1085/jgp.79.2.313
dc.identifier.urihttp://hdl.handle.net/11603/13937
dc.language.isoen_USen_US
dc.publisherRockefeller University Pressen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectcrayfish photoreceptorsen_US
dc.subjectcrayfish rhodopsinen_US
dc.subjectmetarhodopsin fluorescenceen_US
dc.subjectphotoequilibriumen_US
dc.titlePhotosensitivity spectrum of crayfish rhodopsin measured using fluorescence of metarhodopsinen_US
dc.typeTexten_US

Files

License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.56 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UMBC Biological Sciences Department