Heterologous Exchanges of Glycoprotein and Non-Virion Protein in Novirhabdoviruses: Assessment of Virulence in Yellow Perch (Perca flavescens) and Rainbow Trout (Oncorhynchus mykiss)

dc.contributor.authorVakharia, Vikram
dc.contributor.authorAmmayappan, Arun
dc.contributor.authorYusuff, Shamila
dc.contributor.authorTesfaye, Tarin M.
dc.contributor.authorKurath, Gael
dc.date.accessioned2024-05-13T19:11:05Z
dc.date.available2024-05-13T19:11:05Z
dc.date.issued2024-04-22
dc.description.abstractInfectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicemia virus (VHSV) are rhabdoviruses in two different species belonging to the Novirhabdovirus genus. IHNV has a narrow host range restricted to trout and salmon species, and viruses in the M genogroup of IHNV have high virulence in rainbow trout (Oncorhynchus mykiss). In contrast, the VHSV genotype IVb that invaded the Great Lakes in the United States has a broad host range, with high virulence in yellow perch (Perca flavescens), but not in rainbow trout. By using reverse-genetic systems of IHNV-M and VHSV-IVb strains, we generated six IHNV:VHSV chimeric viruses in which the glycoprotein (G), non-virion-protein (NV), or both G and NV genes of IHNV-M were replaced with the analogous genes from VHSV-IVb, and vice versa. These chimeric viruses were used to challenge groups of rainbow trout and yellow perch. The parental recombinants rIHNV-M and rVHSV-IVb were highly virulent in rainbow trout and yellow perch, respectively. Parental rIHNV-M was avirulent in yellow perch, and chimeric rIHNV carrying G, NV, or G and NV genes from VHSV-IVb remained low in virulence in yellow perch. Similarly, the parental rVHSV-IVb exhibited low virulence in rainbow trout, and chimeric rVHSV with substituted G, NV, or G and NV genes from IHNV-M remained avirulent in rainbow trout. Thus, the G and NV genes of either virus were not sufficient to confer high host-specific virulence when exchanged into a heterologous species genome. Some exchanges of G and/or NV genes caused a loss of host-specific virulence, providing insights into possible roles in viral virulence or fitness, and interactions between viral proteins.
dc.description.sponsorshipPart of the study was funded by NSF grant DBI-1354684 (V.N.V). Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.
dc.description.urihttps://www.mdpi.com/1999-4915/16/4/652
dc.format.extent16 pages
dc.genrejournal articles
dc.identifierdoi:10.13016/m2aykz-kfzd
dc.identifier.citationVakharia, Vikram N., Arun Ammayappan, Shamila Yusuff, Tarin M. Tesfaye, and Gael Kurath. “Heterologous Exchanges of Glycoprotein and Non-Virion Protein in Novirhabdoviruses: Assessment of Virulence in Yellow Perch (Perca Flavescens) and Rainbow Trout (Oncorhynchus Mykiss).” Viruses 16, no. 4 (April 2024): 652. https://doi.org/10.3390/v16040652.
dc.identifier.urihttps://doi.org/10.3390/v16040652
dc.identifier.urihttp://hdl.handle.net/11603/33926
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Department of Marine Biotechnology
dc.relation.ispartofUMBC Student Collection
dc.rightsThis work was written as part of one of the author's official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.
dc.rightsPublic Domain
dc.rights.urihttps://creativecommons.org/publicdomain/mark/1.0/
dc.subjectglycoprotein
dc.subjectIHNV
dc.subjectnon-virion protein
dc.subjectrhabdovirus
dc.subjectVHSV
dc.subjectviral pathogenesis
dc.titleHeterologous Exchanges of Glycoprotein and Non-Virion Protein in Novirhabdoviruses: Assessment of Virulence in Yellow Perch (Perca flavescens) and Rainbow Trout (Oncorhynchus mykiss)
dc.typeText
dcterms.creatorhttps://orcid.org/0000-0002-0955-3010
dcterms.creatorhttps://orcid.org/0000-0002-0453-7310

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