Minimally invasive technique for measuring transdermal glucose with a fluorescent biosensor

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dc.contributor.authorBrown, Sheniqua
dc.contributor.authorZambrana, Paige N.
dc.contributor.authorGe, Xudong
dc.contributor.authorBagdure, Dayanand
dc.contributor.authorStinchcomb, Audra L.
dc.contributor.authorRao, Govind
dc.contributor.authorTolosa, Leah
dc.date.accessioned2018-10-30T16:04:38Z
dc.date.available2018-10-30T16:04:38Z
dc.date.issued2018-08-31
dc.description.abstractThere is a need for blood glucose monitoring techniques that eliminate the painful and invasive nature of current methods, while maintaining the reliability and accuracy of established medical technology. This research aims to ultimately address these shortcomings in critically ill pediatric patients. Presented in this work is an alternative, minimally invasive technique that uses microneedles (MN) for the collection of transdermal glucose (TG). Due to their comparable skin properties, diffusion studies were performed on full thickness Yucatan miniature pig skin mounted to an in-line diffusion flow cell and on different skin sites of human subjects. Collected TG samples were measured with a L255C mutant of the E. coli glucose-binding protein (GBP) with an attached fluorescent probe. The binding constant (Kd = 0.67 μM) revealed the micromolar sensitivity and high selectivity of the his-tagged GBP biosensor for glucose, making it suitable for TG measurements. In both the animal and human models, skin permeability and TG diffusion across the skin increased with MN application. For intact and MN-treated human skin, a significant positive linear correlation (r > 0.95, p < 0.01) existed between TG and BG. The micromolar sensitivity of GBP minimized the volume required for interstitial fluid glucose analysis allowing MN application time (30 s) to be shortened compared to other studies. This time reduction can help in eliminating skin irritation issues and improving practical use of the technique by caregivers in the hospital. In addition, the his-tagged optical biosensor used in this work can be immobilized and used with a portable sensing fluorometer device at the point of care (POC) making this minimally invasive technology more ideal for use in the pediatric intensive care unit.en_US
dc.description.sponsorshipThe authors acknowledge the Eunice Kennedy Shriver National Institute of Child Health and Human Development for grant R41HD088223 and the UMB-UMBC Research and Innovation Partnership Grant Program for project funding, as well as, the UMBC Meyerhoff Graduate Program and LSAMP Bridges to the Doctorate Program for S. Brown’s funding. The authors would like to thank Dr. Russel Potts for invaluable input and Eric Ankers for assistance with experimental work.en_US
dc.description.urihttps://link.springer.com/content/pdf/10.1007%2Fs00216-018-1336-8.pdfen_US
dc.format.extent12 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/M22N4ZN4C
dc.identifier.citationSheniqua Brown, Paige N. Zambrana, Xudong Ge, Dayanand Bagdure, Audra L. Stinchcomb, Govind Rao, Leah Tolosa, Minimally invasive technique for measuring transdermal glucose with a fluorescent biosensor, Analytical and Bioanalytical Chemistry November 2018, Volume 410, Issue 27, pp 7249–7260, https://doi.org/10.1007/s00216-018-1336-8en_US
dc.identifier.urihttps://doi.org/10.1007/s00216-018-1336-8
dc.identifier.urihttp://hdl.handle.net/11603/11786
dc.language.isoen_USen_US
dc.publisherSpringer Nature Switzerland AG.en_US
dc.relation.ispartofUMBC Center for Advanced Sensor Technology (CAST)
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectOptical biosensoren_US
dc.subjectIn-line flow through diffusion cellen_US
dc.subjectTransdermal glucose monitoringen_US
dc.subjectMicroneedlesen_US
dc.subjectUMBC Ebiquity Research Groupen_US
dc.titleMinimally invasive technique for measuring transdermal glucose with a fluorescent biosensoren_US
dc.typeTexten_US

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