Altered regulation of the glnRA operon in a Bacillus subtilis mutant that produces methionine sulfoximine-tolerant glutamine synthetase

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dc.contributor.authorH. J., Schreier
dc.contributor.authorC. A., Rostkowski
dc.contributor.authorE. M., Kellner
dc.date.accessioned2019-03-15T19:08:11Z
dc.date.available2019-03-15T19:08:11Z
dc.date.issued1993-02
dc.description.abstractA Bacillus subfilis mutant that produced glutamine synthetase (GS) with altered sensitivity to DL-methionine sulfoximine was isolated. The mutation, designated glnA33, was due to a T- A-to-C- G transition, changing valine to alanine at codon 190 within the active-site C domain. Altered regulation was observed for GS activity and antigen and mRNA levels in a B. subtilis glnA33 strain. The mutant enzyme was 28-fold less sensitive to DL-methionine sulfoximine and had a 13.0-fold-higher Km for hydroxylamine and a 4.8-fold-higher Km for glutamate than wild-type GS did.en
dc.description.sponsorshipThis work was supported by Public Health Service grant GM39541 to H.J.S. from the National Institutes of Health.en
dc.description.urihttps://www.ncbi.nlm.nih.gov/pubmed/8093698en
dc.format.extent6 pagesen
dc.genrejournal articlesen
dc.identifierdoi:10.13016/m2qtgc-o6wm
dc.identifier.citationH J Schreier, C A Rostkowski, E M Kelln, Altered regulation of the glnRA operon in a Bacillus subtilis mutant that produces methionine sulfoximine-tolerant glutamine synthetase, J Bacteriol. 1993 Feb;175(3):892-7, DOI: 10.1128/jb.175.3.892-897.1993en
dc.identifier.urihttps://doi.org/10.1128/jb.175.3.892-897.1993
dc.identifier.urihttp://hdl.handle.net/11603/13075
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Department of Marine Biotechnology
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectBacillus subfilisen
dc.subjectglutamine synthetase (GS)en
dc.subjectDL-methionine sulfoximineen
dc.subjectLmethionine-SR-sulfoximine (MSX)en
dc.titleAltered regulation of the glnRA operon in a Bacillus subtilis mutant that produces methionine sulfoximine-tolerant glutamine synthetaseen
dc.typeTexten

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