Alternate pathways for processing in the internal transcribed spacer 1 in pre-rRNA of Saccharomyces cerevisiae

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dc.contributor.authorLindahl, L.
dc.contributor.authorArcher, R. H.
dc.contributor.authorZengel, J. M.
dc.date.accessioned2019-06-06T17:01:28Z
dc.date.available2019-06-06T17:01:28Z
dc.date.issued1994-12-11
dc.description.abstractWe have extended the system of Nogi et al. (Proc. Natl. Acad. Sci. USA 88, 1991, 3962-3966) for transcription of rRNA from an RNA polymerase II promoter in strains lacking functional RNA polymerase I. In our strains two differentially marked rRNA transcription units can be expressed alternately. Using this system we have shown that the A2 processing site in the internal transcribed spacer 1 (ITS1) of the pre-rRNA is dispensable. According to the accepted processing scheme, the A2 site serves to separate the parts of the primary rRNA transcript that are destined for incorporation into the two ribosomal subunits. However, we have found that, when A2 is impaired, separation of the small and large subunit rRNAs occurs at a processing site further downstream in ITS1, indicating that alternate pathways for ITS1 processing exist. Short deletions in the A2 region still allow residual processing at the A2 site. Mapping of the cleavage sites in such deletion transcripts suggests that sequences downstream of the A2 site are used for determining the position of the cleavage.en_US
dc.description.sponsorshipThis work was supported by a grant from the National Institute of Allergy and Infectious Diseases.en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC332089/en_US
dc.format.extent9 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2nu8r-vb63
dc.identifier.citationL Lindahl, R H Archer, and J M Zengel, Alternate pathways for processing in the internal transcribed spacer 1 in pre-rRNA of Saccharomyces cerevisiae, Nucleic Acids Res. 1994 Dec 11; 22(24): 5399–5407. DOI: 10.1093/nar/22.24.5399en_US
dc.identifier.urihttps://dx.doi.org/10.1093%2Fnar%2F22.24.5399
dc.identifier.urihttp://hdl.handle.net/11603/14031
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectRNA polymeraseen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectrRNA transcripten_US
dc.titleAlternate pathways for processing in the internal transcribed spacer 1 in pre-rRNA of Saccharomyces cerevisiaeen_US
dc.typeTexten_US

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