Interactions among the protein and RNA subunits of Saccharomyces cerevisiae nuclear RNase P

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dc.contributor.authorHouser-Scott, Felicia
dc.contributor.authorXiao, Shaohua
dc.contributor.authorMillikin, Christopher E.
dc.contributor.authorZengel, Janice M.
dc.contributor.authorLindahl, Lasse
dc.contributor.authorEngelke, David R.
dc.date.accessioned2019-06-19T17:05:18Z
dc.date.available2019-06-19T17:05:18Z
dc.date.issued2002-03-05
dc.description.abstractRibonuclease P (RNase P) is a ubiquitous endoribonuclease that cleaves precursor tRNAs to generate mature 5′ termini. Although RNase P from all kingdoms of life have been found to have essential RNA subunits, the number and size of the protein subunits ranges from one small protein in bacteria to at least nine proteins of up to 100 kDa. In Saccharomyces cerevisiae nuclear RNase P, the enzyme is composed of ten subunits: a single RNA and nine essential proteins. The spatial organization of these components within the enzyme is not yet understood. In this study we examine the likely binary protein–protein and protein–RNA subunit interactions by using directed two- and three-hybrid tests in yeast. Only two protein subunits, Pop1p and Pop4p, specifically bind the RNA subunit. Pop4p also interacted with seven of the other eight protein subunits. The remaining protein subunits all showed one or more specific protein–protein interactions with the other integral protein subunits. Of particular interest was the behavior of Rpr2p, the only protein subunit found in RNase P but not in the closely related enzyme, RNase MRP. Rpr2p interacts strongly with itself as well as with Pop4p. Similar interactions with self and Pop4p were also detected for Snm1p, the only unique protein subunit so far identified in RNase MRP. This observation is consistent with Snm1p and Rpr2p serving analogous functions in the two enzymes. This study provides a low-resolution map of the multisubunit architecture of the ribonucleoprotein enzyme, nuclear RNase P from S. cerevisiae.en_US
dc.description.sponsorshipFunding for this work was provided by National Institutes of Health Grant GM34869 (to D.R.E.), National Science Foundation Grant MCB-0077949 (to L.L. and J.M.Z.), and postdoctoral fellowships to F.H.-S. from United Negro College Fund/Pfizer and the Ford Foundation.en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC122408/en_US
dc.format.extent6 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2l5a7-q2yu
dc.identifier.citationFelicia Houser-Scott, et.al, Interactions among the protein and RNA subunits of Saccharomyces cerevisiae nuclear RNase P, Proc Natl Acad Sci U S A. 2002 Mar 5; 99(5): 2684–2689. DOI: 10.1073/pnas.052586299en_US
dc.identifier.urihttps://dx.doi.org/10.1073%2Fpnas.052586299
dc.identifier.urihttp://hdl.handle.net/11603/14269
dc.language.isoen_USen_US
dc.publisherNational Academy of Sciencesen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectRibonuclease Pen_US
dc.subjectendoribonucleaseen_US
dc.titleInteractions among the protein and RNA subunits of Saccharomyces cerevisiae nuclear RNase Pen_US
dc.typeTexten_US

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