Novel Calcium Phosphate Cement with Metformin-Loaded Chitosan for Odontogenic Differentiation of Human Dental Pulp Cells

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dc.contributorKim, Howard
dc.contributor.authorQin, Wei
dc.contributor.authorChen, Jia-Yao
dc.contributor.authorGuo, Jia
dc.contributor.authorMa, Tao
dc.contributor.authorWeir, Michael D.
dc.contributor.authorGuo, Dong
dc.contributor.authorShu, Yan
dc.contributor.authorLin, Zheng-Mei
dc.contributor.authorSchneider, Abraham
dc.contributor.authorH. K. Xu, Hockin
dc.date.accessioned2018-12-18T15:32:55Z
dc.date.available2018-12-18T15:32:55Z
dc.date.issued2018-11-27
dc.description.abstractMetformin is an old and widely accepted first-line drug for treating type 2 diabetes. Our previous studies demonstrate that metformin can stimulate the osteo/odontogenic differentiation of human-induced pluripotent stem cell-derived mesenchymal stem cells and human dental pulp cells (DPCs). Due to the rapid dilution of metformin from the defect area, the aim of this study was to develop a drug delivery system with controlled release of metformin to promote cell viability and odontogenic differentiation of DPCs favoring dentin regeneration. Calcium phosphate cement (CPC) containing chitosan and metformin as a scaffold was synthesized. DPCs were seeded onto the scaffold, and the viability and proliferation were evaluated at several time points. For osteogenic differentiation analysis, alkaline phosphatase (ALP) activity was tested, cells were stained with Alizarin Red, and the expression of odontogenic markers was evaluated by real-time polymerase chain reaction. DPCs remained viable and attached well to the CPC-chitosan composite scaffold. Moreover, the addition of metformin to the CPC-chitosan composite did not adversely affect cell proliferation, compared to that of CPC control. Our data further revealed that the novel CPC-chitosan-metformin composite enhanced the odontogenic differentiation of DPCs, as evidenced by higher ALP activity, elevated expression of odontoblastic markers, and strong mineral deposition. These results suggest that the new CPC-chitosan-metformin composite is a highly promising scaffold with the potential for tissue engineering applications including dentin regeneration.en_US
dc.description.sponsorshipThis study was supported by the National Institutes of Health/National Institute of Dental and Craniofacial Research Grant R01 DE023578 (to AS).en_US
dc.description.urihttps://www.hindawi.com/journals/sci/2018/7173481/abs/en_US
dc.format.extent11 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/M2RN30C1K
dc.identifier.citationWei Qin, Jia-Yao Chen, Jia Guo, Tao Ma, Michael D. Weir, Dong Guo, Yan Shu, Zheng-Mei Lin, Abraham Schneider, and Hockin H. K. Xu, Novel Calcium Phosphate Cement with Metformin-Loaded Chitosan for Odontogenic Differentiation of Human Dental Pulp Cells, Stem Cells International Volume 2018, Article ID 7173481, 10 pages https://doi.org/10.1155/2018/7173481en_US
dc.identifier.urihttps://doi.org/10.1155/2018/7173481
dc.identifier.urihttp://hdl.handle.net/11603/12291
dc.language.isoen_USen_US
dc.publisherHindawien_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Mechanical Engineering Department Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution 4.0 International (CC BY 4.0)*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectcalcium phosphateen_US
dc.subjectcementen_US
dc.subjectodontogenicen_US
dc.subjecthuman dental pulp cellsen_US
dc.subjectmetformin-loaded chitosanen_US
dc.titleNovel Calcium Phosphate Cement with Metformin-Loaded Chitosan for Odontogenic Differentiation of Human Dental Pulp Cellsen_US
dc.typeTexten_US

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