Enhancement of human neural stem cell self‐renewal in 3D hypoxic culture

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dc.contributor.authorGhourichaee, Sasan Sharee
dc.contributor.authorPowell, Elizabeth M.
dc.contributor.authorLeach, Jennie B.
dc.date.accessioned2018-12-17T15:16:46Z
dc.date.available2018-12-17T15:16:46Z
dc.date.issued2016-11-21
dc.description.abstractThe pathology of neurological disorders is associated with the loss of neuronal and glial cells that results in functional impairments. Human neural stem cells (hNSCs), due to their self-renewing and multipotent characteristics, possess enormous tissue-specific regenerative potential. However, the efficacy of clinical applications is restricted due to the lack of standardized in vitro cell production methods with the capability of generating hNSC populations with well-defined cellular compositions. At any point, a population of hNSCs may include undifferentiated stem cells, intermediate and terminally differentiated progenies, and dead cells. Due to the plasticity of hNSCs, environmental cues play crucial roles in determining the cellular composition of hNSC cultures over time. Here, we investigated the independent and synergistic effect of three important environmental factors (i.e., culture dimensionality, oxygen concentration, and growth factors) on the survival, renewal potential, and differentiation of hNSCs. Our experimental design included two dimensional (2D) versus three dimensional (3D) cultures and normoxic (21% O₂ ) versus hypoxic (3% O₂ ) conditions in the presence and absence of epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2). Additionally, we discuss the feasibility of mathematical models that predict hNSC growth and differentiation under these culture conditions by adopting a negative feedback regulatory term. Our results indicate that the synergistic effect of culture dimensionality and hypoxic oxygen concentration in the presence of growth factors enhances the proliferation of viable, undifferentiated hNSCs. Moreover, the same synergistic effect in the absence of growth factors promotes the differentiation of hNSCs. Biotechnol. Bioeng. 2017;114: 1096-1106.en_US
dc.description.sponsorshipThe authors acknowledge support for this work from the National Science Foundation (CBET 1447057; JL), Maryland Stem Cell Research Fund (2009‐MSCRFE‐0039‐00; EMP and JL), UMBC (JL), and a UMBC Dissertation Fellowship (SG).en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pubmed/27869294en_US
dc.format.extent11 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/M2ZC7RZ6B
dc.identifier.citationSasan Sharee Ghourichaee, Elizabeth M. Powell, Jennie B. Leach,Enhancement of human neural stem cell self‐renewal in 3D hypoxic culture, Biotechnology and Bioengineering, Volume 114, Issue 5 ,https://doi.org/10.1002/bit.26224en_US
dc.identifier.urihttps://doi.org/10.1002/bit.26224
dc.identifier.urihttp://hdl.handle.net/11603/12274
dc.language.isoen_USen_US
dc.publisherWiley Periodicalsen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subject3D cultureen_US
dc.subjecthuman neural stem cellsen_US
dc.subjectproliferationen_US
dc.subjectself-renewalen_US
dc.subjectviabilityen_US
dc.titleEnhancement of human neural stem cell self‐renewal in 3D hypoxic cultureen_US
dc.typeTexten_US

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