Luminescent quantum dots for cellular analysis

Author/Creator ORCID





Citation of Original Publication

Rosenzweig, Zeev; Shi, Lifang; Rosenzweig, Nitsa; Luminescent quantum dots for cellular analysis; Proceedings Volume 6370, Nanomaterial Synthesis and Integration for Sensors, Electronics, Photonics, and Electro-Optics; 637003 (2006);


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The paper describes the fabrication, characterization and applications of novel luminescent quantum dots fluorescence resonance energy transfer (FRET) based enzymatic activity probes. The luminescent probes are based on FRET between luminescent quantum dots that serve as donors and rhodamine acceptors that are immobilized to the surface of the quantum dots through peptide linkers that contain selective enzymatic cleavage sites. Upon enzymatic cleavage of the peptide linkers the rhodamine molecules no longer provide an efficient energy transfer channel to the quantum dots, which lightes up the initially quenched the quantum dots. The quantum dots based probes were applied for detecting enzyme activity and screening enzyme inhibitors. They were also used for the measurement of extracellular matrix metallproteinases (MMPs) activity in normal and cancerous breast cells tissues.