Reporter Scaffolds for Clinically Relevant Cell Transplantation Studies

Simple item page
dc.contributor.authorBolger, Morgan
dc.contributor.authorGroynom, Rebecca
dc.contributor.authorBogie, Kath
dc.contributor.authorLavik, Erin
dc.date.accessioned2020-01-28T16:52:12Z
dc.date.available2020-01-28T16:52:12Z
dc.date.issued2019-11-04
dc.description.abstractThere are a number of cell therapies that are either in clinical trials or moving toward clinical trials, particularly for diseases of the retina. One of the challenges with cell therapies is tracking the status of cells over time. Genetic manipulation can facilitate this, but it can limit the clinical application of the cells. There are a host of fluorophores that have been developed to assess the status of cells, but these molecules tend to be cleared rapidly from cells. There are preclinical strategies that use degradable scaffolds, and we hypothesized that these scaffolds could be used to track the state of cells during preclinical studies. In this work, we explored whether fluorophores could be delivered from simple scaffolds fabricated under extremely harsh conditions, be active upon release, and report on the cells growing on the scaffolds over time. We encapsulated CellROX® Green Reagent, and pHrodo™ Red AM in poly(lactic-co-glycolic acid) (PLGA) scaffolds, showed that they could be delivered over weeks and were still active upon release and taken up by cells. These experiments provide the foundation for using scaffolds to deliver molecules to report on cells.en_US
dc.description.sponsorshipThis work was supported by a grant from the Steven J. Ryan Initiative for Macular Research and NIH grant 1R56NS100732-01. Dr. Lavik is an inventor on intellectual property that includes the potential for incorporation of reporter molecules.en_US
dc.description.urihttps://link.springer.com/article/10.1007/s10439-019-02393-zen_US
dc.format.extent13 pagesen_US
dc.genrejournal articles postprintsen_US
dc.identifierdoi:10.13016/m26yq5-ot8y
dc.identifier.citationBolger, Morgan; Groynom, Rebecca; Bogie, Kath; Lavik, Erin; Reporter Scaffolds for Clinically Relevant Cell Transplantation Studies; Annals of Biomedical Engineering (2019); https://link.springer.com/article/10.1007/s10439-019-02393-zen_US
dc.identifier.urihttps://doi.org/10.1007/s10439-019-02393-z
dc.identifier.urihttp://hdl.handle.net/11603/17132
dc.language.isoen_USen_US
dc.publisherSpringer USen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsThis is a post-peer-review, pre-copyedit version of an article published in Annals of Biomedical Engineering. The final authenticated version is available online at: https://doi.org/10.1007/s10439-019-02393-z.
dc.rightsAccess to this item will begin on 2020-11-04
dc.subjectpolymeren_US
dc.subjectretinaen_US
dc.subjectage related macular degenerationen_US
dc.subjectAMDen_US
dc.subjectcellular transplantationen_US
dc.subjecttissue engineeringen_US
dc.subjectscaffolden_US
dc.subjectpolyesteren_US
dc.subjectdrug deliveryen_US
dc.titleReporter Scaffolds for Clinically Relevant Cell Transplantation Studiesen_US
dc.typeTexten_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
reporter-scaffold-paper-revision (mwb comments).pdf
Size:
527.69 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.56 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UMBC Chemical, Biochemical & Environmental Engineering Department
UMBC Faculty Collection