Electrospun Microfibers Modulate Intracellular Amino Acids in Liver Cells via Integrin β1

dc.contributor.authorHuang, Tianjiao
dc.contributor.authorTerrell, John A.
dc.contributor.authorChung, Jay H.
dc.contributor.authorChen, Chengpeng
dc.date.accessioned2022-02-07T19:11:40Z
dc.date.available2022-02-07T19:11:40Z
dc.date.issued2021-05-14
dc.description.abstractAlthough numerous recent studies have shown the importance of polymeric microfibrous extracellular matrices (ECMs) in maintaining cell behaviors and functions, the mechanistic nexus between ECMs and intracellular activities is largely unknown. Nevertheless, this knowledge will be critical in understanding and treating diseases with ECM remodeling. Therefore, we present our findings that ECM microstructures could regulate intracellular amino acid levels in liver cells mechanistically through integrin β1. Amino acids were studied because they are the fundamental blocks for protein synthesis and metabolism, two vital functions of liver cells. Two ECM conditions, flat and microfibrous, were prepared and studied. In addition to characterizing cell growth, albumin production, urea synthesis, and cytochrome p450 activity, we found that the microfibrous ECM generally upregulated the intracellular amino acid levels. Further explorations showed that cells on the flat substrate expressed more integrin β1 than cells on the microfibers. Moreover, after partially blocking integrin β1 in cells on the flat substrate, the intracellular amino acid levels were restored, strongly supporting integrin β1 as the linking mechanism. This is the first study to report that a non-biological polymer matrix could regulate intracellular amino acid patterns through integrin. The results will help with future therapy development for liver diseases with ECM changes (e.g., fibrosis).en_US
dc.description.urihttps://www.mdpi.com/2306-5354/8/7/88en_US
dc.format.extent12 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2abbf-9bvw
dc.identifier.citationHuang, Tianjiao, John A. Terrell, Jay H. Chung, and Chengpeng Chen. 2021. "Electrospun Microfibers Modulate Intracellular Amino Acids in Liver Cells via Integrin β1" Bioengineering 8, no. 7: 88. https://doi.org/10.3390/bioengineering8070088en_US
dc.identifier.urihttps://doi.org/10.3390/bioengineering8070088
dc.identifier.urihttp://hdl.handle.net/11603/24130
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemistry & Biochemistry Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis work was written as part of one of the author's official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.en_US
dc.rightsPublic Domain Mark 1.0*
dc.rights.urihttp://creativecommons.org/publicdomain/mark/1.0/*
dc.titleElectrospun Microfibers Modulate Intracellular Amino Acids in Liver Cells via Integrin β1en_US
dc.typeTexten_US
dcterms.creatorhttps://orcid.org/0000-0001-7754-344Xen_US

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