Set of Highly Stable Amine- and Carboxylate-Terminated Dendronized Au Nanoparticles with Dense Coating and Nontoxic Mixed-Dendronized Form

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dc.contributor.authorRay, Arunendra Saha
dc.contributor.authorGhann, William E.
dc.contributor.authorTsoi, Phoebe S.
dc.contributor.authorSzychowski, Brian
dc.contributor.authorDockery, Lance T.
dc.contributor.authorPak, Yewon J.
dc.contributor.authorLi, Wenjing
dc.contributor.authorKane, Maureen A.
dc.contributor.authorSwaan, Peter
dc.contributor.authorDaniel, Marie-Christine
dc.date.accessioned2019-02-19T16:08:27Z
dc.date.available2019-02-19T16:08:27Z
dc.date.issued2019-02-04
dc.description.abstractThe synthesis of a novel poly(propyleneimine) (PPI) dendron in gram scale as well as its use in the formation of a highly stable, dendronized gold nanoparticle (AuNP)-based drug delivery platform is described herein. The AuNP-based platform is composed of three complementary parts: (i) a 15 nm AuNP core, (ii) a heterofunctional thioctic acid-terminated tetraethylene glycol spacer, and (iii) a third-generation PPI dendron with a unique protonation profile and diverse end-group functionalization that allows for further derivatization. The prepared dendronized AuNPs are able to withstand several rounds of lyophilization cycles with no sign of aggregation, are stable in phosphate-buffered saline and Hanks’ buffer as well as in serum, and are resistant to degradation by glutathione exchange reactions. This nanocarrier platform displays a dense coating, with >1400 dendrons/AuNPs, which will enable very high payload. Furthermore, while amine-terminated AuNPs expectedly showed cytotoxicity against the MCF-7 breast cancer cell line from a NP concentration of 1 nM, the mixed monolayer AuNPs (coated with 40/60 amine/carboxylate dendrons) interestingly did not exhibit any sign of toxicity at concentrations as high as 15 nM, similar to the carboxylate-terminated AuNPs. The described dendronized AuNPs address the current practical need for a stable NP-based drug delivery platform which is scalable and easily conjugable, has long-term stability in solution, and can be conveniently formulated as a powder and redispersed in desired buffer or serum.en_US
dc.description.sponsorshipThe authors are grateful for the financial support from NSF (CHE1507462) and NIH (T32GM066706). They would also like to acknowledge Josh Wilhide (MCAC, UMBC) for assistance in acquisition of HRMS spectra. They are also thankful to Tagide deCarvalho for her assistance in sample preparation and acquisition of TEM images, as well to Laszlo Takacs for his assistance in acquisition of STEM images. Additional support was provided by the University of Maryland School of Pharmacy Mass Spectrometry Center (SOP1841-IQB2014).en_US
dc.description.urihttps://pubs.acs.org/doi/10.1021/acs.langmuir.8b03196en_US
dc.format.extent13 pagesen_US
dc.genrejournal articles postprintsen_US
dc.identifierdoi:10.13016/m2spvh-nj1m
dc.identifier.citationArunendra Saha Ray, William E. Ghann, Phoebe S. Tsoi, Brian Szychowski, Lance T. Dockery, Yewon J. Pak, Wenjing Li, Maureen A. Kane, Peter Swaan, and Marie-Christine Daniel, Set of Highly Stable Amine- and Carboxylate-Terminated Dendronized Au Nanoparticles with Dense Coating and Nontoxic Mixed-Dendronized Form, Langmuir Article ASAP, DOI: 10.1021/acs.langmuir.8b03196en_US
dc.identifier.urihttps://doi.org/10.1021/acs.langmuir.8b03196
dc.identifier.urihttp://hdl.handle.net/11603/12819
dc.language.isoen_USen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemistry & Biochemistry Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Langmuir, Article ASAP, ACS Journals, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.langmuir.8b03196
dc.rightsAccess to this item will begin on 02/04/2020
dc.subjectnovel poly(propyleneimine)en_US
dc.subjectgold nanoparticleen_US
dc.subjectderivatizationen_US
dc.subjectnanocarrieren_US
dc.titleSet of Highly Stable Amine- and Carboxylate-Terminated Dendronized Au Nanoparticles with Dense Coating and Nontoxic Mixed-Dendronized Formen_US
dc.typeTexten_US

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