Non-canonical LexA proteins regulate the SOS response in the Bacteroidetes

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dc.contributor.authorSánchez-Osuna, Miquel
dc.contributor.authorCortés, Pilar
dc.contributor.authorLee, Mark
dc.contributor.authorSmith, Aaron T.
dc.contributor.authorBarbe, Jordi
dc.contributor.authorErill, Ivan
dc.date.accessioned2021-11-04T14:43:15Z
dc.date.available2021-11-04T14:43:15Z
dc.date.issued2021-10-06
dc.description.abstractLesions to DNA compromise chromosome integrity, posing a direct threat to cell survival. The bacterial SOS response is a widespread transcriptional regulatory mechanism to address DNA damage. This response is coordinated by the LexA transcriptional repressor, which controls genes involved in DNA repair, mutagenesis and cell-cycle control. To date, the SOS response has been characterized in most major bacterial groups, with the notable exception of the Bacteroidetes. No LexA homologs had been identified in this large, diverse and ecologically important phylum, suggesting that it lacked an inducible mechanism to address DNA damage. Here, we report the identification of a novel family of transcriptional repressors in the Bacteroidetes that orchestrate a canonical response to DNA damage in this phylum. These proteins belong to the S24 peptidase family, but are structurally different from LexA. Their N-terminal domain is most closely related to CI-type bacteriophage repressors, suggesting that they may have originated from phage lytic phase repressors. Given their role as SOS regulators, however, we propose to designate them as non-canonical LexA proteins. The identification of a new class of repressors orchestrating the SOS response illuminates long-standing questions regarding the origin and plasticity of this transcriptional network.en_US
dc.description.sponsorshipSpanish Ministerio de Econom´ıa y Competitividad [BIO2016-77011-R to J.B.]; National Science Foundation (NSF) [CHE1844624 to A.T.S]. M.S.-O. was the recipient of a predoctoral fellowship from the Ministerio de Educacion, Cultura y Deporte de Espa ´ na. Funding for ˜ open access charge: Spanish Ministerio de Econom´ıa y Competitividad.en_US
dc.description.urihttps://academic.oup.com/nar/article/49/19/11050/6382392en_US
dc.format.extent2 filesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2tqgk-mnhx
dc.identifier.citationSánchez-Osuna, Miquel et al.; Non-canonical LexA proteins regulate the SOS response in the Bacteroidetes; Nucleic Acids Research, Volume 49, Issue 19, Pages 11050–11066, 6 October, 2021; https://doi.org/10.1093/nar/gkab773en_US
dc.identifier.urihttps://doi.org/10.1093/nar/gkab773
dc.identifier.urihttp://hdl.handle.net/11603/23216
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemistry & Biochemistry Department Collection
dc.relation.ispartofUMBC Biological Sciences Department
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Computer Science and Electrical Engineering Department
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.titleNon-canonical LexA proteins regulate the SOS response in the Bacteroidetesen_US
dc.typeTexten_US
dcterms.creatorhttps://orcid.org/0000-0003-0660-7359en_US
dcterms.creatorhttps://orcid.org/0000-0002-9332-8683en_US
dcterms.creatorhttps://orcid.org/0000-0002-7280-7191en_US

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