Phylogenetic Analysis of Microbial Communities in Different Regions of the Gastrointestinal Tract in Panaque nigrolineatus, a Wood-Eating Fish
| dc.contributor | Ravel, Jacques | |
| dc.contributor.author | McDonald, Ryan | |
| dc.contributor.author | Schreier, Harold J. | |
| dc.contributor.author | Watts, Joy E. M. | |
| dc.date.accessioned | 2019-03-15T16:30:11Z | |
| dc.date.available | 2019-03-15T16:30:11Z | |
| dc.date.issued | 2012-10-25 | |
| dc.description.abstract | The Neotropical detritivorous catfish Panaque nigrolineatus imbibes large quantities of wood as part of its diet. Due to the interest in cellulose, hemi-cellulose and lignin degradation pathways, this organism provides an interesting model system for the detection of novel microbial catabolism. In this study, we characterize the microbial community present in different regions of the alimentary tract of P. nigrolineatus fed a mixed diet of date palm and palm wood in laboratory aquaria. Analysis was performed on 16S rRNA gene clone libraries derived from anterior and posterior regions of the alimentary tract and the auxiliary lobe (AL), an uncharacterized organ that is vascularly attached to the midgut. Sequence analysis and phylogenetic reconstruction revealed distinct microbial communities in each tissue region. The foregut community shared many phylotypes in common with aquarium tank water and included Legionella and Hyphomicrobium spp. As the analysis moved further into the gastrointestinal tract, phylotypes with high levels of 16S rRNA sequence similarity to nitrogen-fixing Rhizobium and Agrobacterium spp. and Clostridium xylanovorans and Clostridium saccharolyticum, dominated midgut and AL communities. However, the hindgut was dominated almost exclusively by phylotypes with the highest 16S rRNA sequence similarity to the Cytophaga-Flavobacterium-Bacteroides phylum. Species richness was highest in the foregut (Chao₁ = 26.72), decreased distally through the midgut (Chao₁ = 25.38) and hindgut (Chao₁ = 20.60), with the lowest diversity detected in the AL (Chao₁ = 18.04), indicating the presence of a specialized microbial community. Using 16S rRNA gene phylogeny, we report that the P. nigrolineatus gastrointestinal tract possesses a microbial community closely related to microorganisms capable of cellulose degradation and nitrogen fixation. Further studies are underway to determine the role of this resident microbial community in Panaque nigrolineatus. | en_US |
| dc.description.sponsorship | This work was supported, in part, by the National Science Foundation, award 0801830 (J.E.M.W. and H.J.S.) and Marie Curie FP7-RG 276948 (J.E.M.W). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | en_US |
| dc.description.uri | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0048018 | en_US |
| dc.format.extent | 9 pages | en_US |
| dc.genre | journal articles | en_US |
| dc.identifier | doi:10.13016/m2safy-qqbv | |
| dc.identifier.citation | McDonald R, Schreier HJ, Watts JEM (2012) Phylogenetic Analysis of Microbial Communities in Different Regions of the Gastrointestinal Tract in Panaque nigrolineatus, a Wood-Eating Fish. PLoS ONE 7(10): e48018. https://doi.org/10.1371/journal.pone.0048018 | en_US |
| dc.identifier.uri | https://doi.org/10.1371/journal.pone.0048018 | |
| dc.identifier.uri | http://hdl.handle.net/11603/13073 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | PLOS | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Biological Sciences Department Collection | |
| dc.relation.ispartof | UMBC Faculty Collection | |
| dc.relation.ispartof | UMBC Department of Marine Biotechnology | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | Attribution 4.0 International (CC BY 4.0) | * |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject | Panaque nigrolineatus | en_US |
| dc.subject | nitrogen-fixing Rhizobium and Agrobacterium spp. | en_US |
| dc.subject | Clostridium xylanovorans | en_US |
| dc.subject | Clostridium saccharolyticum | en_US |
| dc.subject | Loricariidae catfish | en_US |
| dc.title | Phylogenetic Analysis of Microbial Communities in Different Regions of the Gastrointestinal Tract in Panaque nigrolineatus, a Wood-Eating Fish | en_US |
| dc.type | Text | en_US |