Pulsed electrochemical detection in bioanalysis: chemical fingerprinting

Files

103.pdf (304.6 KB)

Links to Files

https://www.spiedigitallibrary.org/conference-proceedings-of-spie/5261/0000/Pulsed-electrochemical-detection-in-bioanalysis-chemical-fingerprinting/10.1117/12.516162.full

Permanent Link

https://doi.org/10.1117/12.516162
 http://hdl.handle.net/11603/40622

Collections

UMBC Faculty Collection
UMBC Chemistry & Biochemistry Department
UMBC Office of the Dean of the College of Natural and Mathematical Sciences

Author/Creator

Author/Creator ORCID

https://orcid.org/0000-0003-2635-9460

Date

2004-03-15

Type of Work

conference papers and proceedings
Text

Department

Program

Citation of Original Publication

LaCourse, William R. “Pulsed Electrochemical Detection in Bioanalysis: Chemical Fingerprinting.” Smart Medical and Biomedical Sensor Technology 5261 (March 2004): 103–12. https://doi.org/10.1117/12.516162.

Rights

©2004 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Subjects

Abstract

Pulsed Electrochemical Detection (PED) is a revolutionary approach to the simple, sensitive, and direct detection of numerous polar aliphatic compounds, especially carbohydrates. This technique exploits the electrocatalytic activity of noble metal electrode surfaces to oxidize various polar functional groups. In PED, multi-step potential-time waveforms at Au and Pt electrodes realize amperometric/coulometric detection while maintaining uniform and reproducible electrode activity. The response mechanisms in PED are dominated by the surface properties of the electrode, and, as a consequence, members of each chemical class of compounds produce virtually identical voltammetric responses. Thus, the full potential is realized when combined with high performance liquid chromatography (HPLC). This paper reviews the fundamental aspects of PED and details a novel approach to the chemical "fingerprinting" of natural products. Applications include the characterization of tobacco, peptones, and bacteria.