Set4 is a chromatin-associated protein, promotes survival during oxidative stress, and regulates stress response genes in yeast

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dc.contributor.authorTran, Khoa
dc.contributor.authorJethmalani, Yogita
dc.contributor.authorJaiswal, Deepika
dc.contributor.authorGreen, Erin
dc.date.accessioned2018-09-18T18:05:34Z
dc.date.available2018-09-18T18:05:34Z
dc.date.issued2018-09
dc.descriptionThis research was originally published in the Journal of Biological Chemistry. Khoa Tran, Yogita Jethmalani, Deepika Jaiswal, Erin M. Green. Set4 is a chromatin-associated protein, promotes survival during oxidative stress, and regulates stress response genes in yeast. J. Biol. Chem. 2018; Vol:293,14429. © Khoa Tran, Yogita Jethmalani, Deepika Jaiswal, and Erin M. Greenen_US
dc.description.abstractThe Set4 protein in the yeast Saccharomyces cerevisiae contains both a PHD finger and a SET domain, a common signature of chromatin-associated proteins, and shares sequence homology with the yeast protein Set3, the fly protein UpSET, and the human protein mixed-lineage leukemia 5 (MLL5). However, the biological role for Set4 and its potential function in chromatin regulation has not been well defined. Here, we analyzed yeast cell phenotypes associated with loss of Set4 or its overexpression, which revealed that Set4 protects against oxidative stress induced by hydrogen peroxide. Gene expression analysis indicated that Set4 promotes the activation of stress response genes in the presence of oxidative insults. Using ChIP analysis and other biochemical assays, we also found that Set4 interacts with chromatin and directly localizes to stress response genes upon oxidative stress. However, recombinant Set4 did not show detectable methyltransferase activity on histones. Our findings also suggest that Set4 abundance in the cell is balanced under normal and stress conditions to promote survival. Overall, these results suggest a model in which Set4 is a stress-responsive, chromatin-associated protein that activates gene expression programs required for cellular protection against oxidative stress. This work advances our understanding of mechanisms that protect cells during oxidative stress and further defines the role of the Set3–Set4 subfamily of SET domain–containing proteins in controlling gene expression in response to adverse environmental conditions.en_US
dc.description.sponsorshipThis work was supported in part by National Institutes of Health Grant R01GM124342 (to E. M. G.). The authors declare that they have no conflicts of interest with the contents of this article. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.en_US
dc.description.urihttps://www.jbc.org/article/S0021-9258(20)30914-5/fulltexten_US
dc.format.extent15 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/M2PR7MZ03
dc.identifier.citationKhoa Tran, Yogita Jethmalani, Deepika Jaiswal, and Erin M. Green Set4 is a chromatin-associated protein, promotes survival during oxidative stress, and regulates stress response genes in yeast, Journal of Biological Chemistry, 2018, 293: 14429 - doi:10.1074/jbc.RA118.003078en_US
dc.identifier.urihttps://doi.org/10.1074/jbc.RA118.003078
dc.identifier.urihttp://hdl.handle.net/11603/11312
dc.language.isoen_USen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item may be protected under Title 17 of the U.S. Copyright Law. It is made available by UMBC for non-commercial research and education. For permission to publish or reproduce, please contact the author.
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectchromatinen_US
dc.subjectchromatin regulationen_US
dc.subjectgene expressionen_US
dc.subjectoxidative stressen_US
dc.subjectstress responseen_US
dc.subjectyeasten_US
dc.subjectgene regulationen_US
dc.subjectSET domainen_US
dc.titleSet4 is a chromatin-associated protein, promotes survival during oxidative stress, and regulates stress response genes in yeasten_US
dc.typeTexten_US
dcterms.creatorhttps://orcid.org/0000-0003-3422-8356
dcterms.creatorhttps://orcid.org/0000-0003-3923-6726

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