Understanding lipogenesis by dynamically profiling transcriptional activity of lipogenic promoters in Yarrowia lipolytica

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dc.contributor.authorLiu, Huan
dc.contributor.authorMarsafari, Monireh
dc.contributor.authorDeng, Li
dc.contributor.authorXu, Peng
dc.date.accessioned2019-02-25T15:11:18Z
dc.date.available2019-02-25T15:11:18Z
dc.date.issued2019-02-07
dc.description.abstractLipogenesis is a complicated process involving global transcriptional reprogramming of lipogenic pathways. It is commonly believed that nitrogen starvation triggers a metabolic shift that reroutes carbon flux from Krebs cycles to lipogenesis. In this study, we systematically surveyed and dynamically profiled the transcriptional activity of 22 lipogenic promoters aiming to delineate a picture how nitrogen starvation regulates lipogenesis in Y. lipolytica. These lipogenic promoters drive the expression of critical pathways that are responsible for the generation of reducing equivalents (NADPH), carbon backbones (acetyl-CoA, malonyl-CoA, DHAP, etc.), synthesis and degradation of fatty acids. Specifically, our investigated promoters span across an array of metabolic pathways, including glycolysis, Krebs cycle, pentose phosphate pathway, mannitol cycle, glutamine–GABA cycle, fatty acid and lipid synthesis, glyoxylate, β-oxidation, and POM (pyruvate–oxaloacetate–malate) cycle. Our work provides evidences that mannitol cycle, glutamine–GABA cycle and amino acid degradation, pyruvate oxidation, and acetate assimilation pathways are lipogenesis-related steps involved in generating cytosolic NADPH and acetyl-CoA precursors. This systematic investigation and dynamic profiling of lipogenic promoters may help us better understand lipogenesis, facilitate the formulation of structure-based kinetic models, as well as develop efficient cell factories for fuels and chemical production in oleaginous species.en_US
dc.description.sponsorshipThis work was supported by the Cellular and Biochemical Engineering Program of the National Science Foundation under grant no.1805139 and the Department of Chemical, Biochemical and Environmental Engineering at University of Maryland Baltimore County.en_US
dc.description.urihttps://link.springer.com/article/10.1007/s00253-019-09664-8en_US
dc.format.extent13 pagesen_US
dc.genrejournal articles postprintsen_US
dc.identifierdoi:10.13016/m2uh8f-x3as
dc.identifier.citationLiu, H., Marsafari, M., Deng, L. et al. ,Understanding lipogenesis by dynamically profiling transcriptional activity of lipogenic promoters in Yarrowia lipolytica, Appl Microbiol Biotechnol (2019). https://doi.org/10.1007/s00253-019-09664-8en_US
dc.identifier.urihttps://doi.org/10.1007/s00253-019-09664-8
dc.identifier.urihttp://hdl.handle.net/11603/12854
dc.language.isoen_USen_US
dc.publisherSpringer Nature Switzerland AG.en_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsThis is a post-peer-review, pre-copyedit version of an article published in Applied Microbiology and Biotechnology. The final authenticated version is available online at: https://doi.org/10.1007/s00253-019-09664-8
dc.rightsAccess to this item will begin on February 7, 2020
dc.subjectoleaginous yeasten_US
dc.subjectlipogenesisen_US
dc.subjectnitrogen starvationsen_US
dc.subjectreducing equivalentsen_US
dc.subjectcarbon backbonesen_US
dc.titleUnderstanding lipogenesis by dynamically profiling transcriptional activity of lipogenic promoters in Yarrowia lipolyticaen_US
dc.typeTexten_US

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