Intermediates in the degradation of mRNA from the lactose operon of Escherichia coli

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dc.contributor.authorMcCormick, Joseph R.
dc.contributor.authorZengel, Janice M.
dc.contributor.authorLindahl, Lasse
dc.date.accessioned2019-06-05T19:15:16Z
dc.date.available2019-06-05T19:15:16Z
dc.date.issued1991-05-25
dc.description.abstractWe have analyzed the processing of mRNA from the lac operon in an Escherichia coli strain carrying the lac on a multicopy plasmid. Messenger RNA was analyzed by hybridization and nuclease protection of pulse-labeled RNA and precursor-product relationships were determined by quantitating radioactivity in primary and processed transcripts at various times after induction of the lac promoter or inhibition of transcription with rifampicin. Our results support the existence of two types of processed transcripts with endpoints in the lacZ-lacY intercistronic region. One of these carries lacZ sequences and has a 3' endpoint about 30 bases downstream of this gene. The other carries lacY sequences and has a 5' end in the translation termination region of the lacZ gene. Finally, we have found evidence that transcription is continued at least 268 bases beyond the last gene (lacA) and that this 3' non-translated region is shortened by post-transcriptional processing.en
dc.description.sponsorshipThis investigation was supported by a research grant and a Research Career Development Award (to LL) from the National Institute of Allergy and Infectious Diseases. JRM was supported, in part, by a predoctoral training grant from the National Institute for General Medicine.en
dc.description.urihttps://www.ncbi.nlm.nih.gov/pubmed/1710346en
dc.format.extent10 pagesen
dc.genrejournal articlesen
dc.identifierdoi:10.13016/m2svoe-hqw0
dc.identifier.citationJoseph R. McCormick Janice M. Zengel Lasse Lindahl, Intermediates in the degradation of mRNA from the lactose operon of Escherichia coli, Nucleic Acids Res. 1991 May 25;19(10):2767-76, https://doi.org/10.1093/nar/19.10.2767en
dc.identifier.urihttps://doi.org/10.1093/nar/19.10.2767
dc.identifier.urihttp://hdl.handle.net/11603/14025
dc.language.isoenen
dc.publisherOxford University Pressen
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectEscherichia colien
dc.subjectprecursor-product relationshipsen
dc.subjectlactose operonen
dc.subjectpost-transcriptional processingen
dc.titleIntermediates in the degradation of mRNA from the lactose operon of Escherichia colien
dc.typeTexten

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