Sequence and Properties of Cagein, a Coiled‐Coil Scaffold Protein Linking Basal Bodies in the Polykinetids of the Ciliate Euplotes aediculatus
| dc.contributor.author | Kloetzel, John A. | |
| dc.contributor.author | Aubusson‐Fleury, Anne | |
| dc.contributor.author | Butler, Maurice D. | |
| dc.contributor.author | Banerjee, Deben | |
| dc.contributor.author | Mozzicafreddo, Matteo | |
| dc.date.accessioned | 2021-03-30T17:26:22Z | |
| dc.date.available | 2021-03-30T17:26:22Z | |
| dc.date.issued | 2021-03-19 | |
| dc.description.abstract | In the hypotrich ciliate Euplotes, many individual basal bodies are grouped together in tightly packed clusters, forming ventral polykinetids. These groups of basal bodies (which produce compound ciliary organelles such as cirri and oral membranelles) are cross‐linked into ordered arrays by scaffold structures known as ‘basal body cages’. The major protein comprising Euplotes cages has been previously identified and termed ‘cagein’. Screening a E. aediculatus cDNA expression library with anti‐cagein antisera identified a DNA insert containing most of a putative cagein gene; standard PCR techniques were used to complete the sequence. Probes designed from this gene identified a macronuclear ‘nano‐chromosome’ of ca. 1.5 kb in Southern blots against whole‐cell DNA. The protein derived from this sequence (463 residues) is predicted to be hydrophilic and highly charged; however, the native cage structures are highly resistant to salt/detergent extraction. This insolubility could be explained by the coiled‐coil regions predicted to extend over much of the length of the derived cagein polypeptide. One frameshift sequence is found within the gene, as well as a short intron. BLAST searches find many ciliates with evident homologues to cagein within their derived genomic sequences. | en_US |
| dc.description.sponsorship | We wish to thank Anne Baroin-Tourancheau (Laboratoire de Biologie Cellulaire 4, Université ParisSud, 91405 Orsay, France) for her indispensible help with expression library screening. The assistance of Marilis Rodriguez and Andrea Molinaro, New York Blood Center, as well as Nick Ambulos, University of Maryland, Baltimore, for DNA sequencing, is gratefully acknowledged. Cristina Miceli (Università di Camerino) aided considerably in sequence analysis. The MARC Program (grant T34 GM136497 from NIGM) provided support for Maurice Butler during his undergraduate research at UMBC. Other support from UMBC included a Designated Research Initiative Fund award to JAK. The residence of JAK in Orsay was supported by a grant from the CNRS, and in Camerino through funds provided by the University of Camerino. | en_US |
| dc.description.uri | https://onlinelibrary.wiley.com/doi/10.1111/jeu.12850 | en_US |
| dc.format.extent | 28 pages | en_US |
| dc.genre | journal articles postprints | en_US |
| dc.identifier | doi:10.13016/m2yqav-5cpo | |
| dc.identifier.citation | Kloetzel, John A.; Aubusson‐Fleury, Anne; Butler, Maurice D.; Banerjee, Deben; Mozzicafreddo, Matteo; Sequence and Properties of Cagein, a Coiled‐Coil Scaffold Protein Linking Basal Bodies in the Polykinetids of the Ciliate Euplotes aediculatus; Eukaryotic Microbiology (2021); https://onlinelibrary.wiley.com/doi/10.1111/jeu.12850 | en_US |
| dc.identifier.uri | https://doi.org/10.1111/jeu.12850 | |
| dc.identifier.uri | http://hdl.handle.net/11603/21256 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Wiley Online Library | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Biological Sciences Department Collection | |
| dc.relation.ispartof | UMBC Faculty Collection | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | This is the peer reviewed version of the following article: Kloetzel, John A.; Aubusson‐Fleury, Anne; Butler, Maurice D.; Banerjee, Deben; Mozzicafreddo, Matteo; Sequence and Properties of Cagein, a Coiled‐Coil Scaffold Protein Linking Basal Bodies in the Polykinetids of the Ciliate Euplotes aediculatus; Eukaryotic Microbiology (2021); https://onlinelibrary.wiley.com/doi/10.1111/jeu.12850 ,which has been published in final form at https://doi.org/10.1111/jeu.12850. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | |
| dc.rights | Access to this item will begin on 2022-03-19 | |
| dc.title | Sequence and Properties of Cagein, a Coiled‐Coil Scaffold Protein Linking Basal Bodies in the Polykinetids of the Ciliate Euplotes aediculatus | en_US |
| dc.type | Text | en_US |