A Modular Toolkit to Fabricate Microfluidic Devices for 3D Cell Culture and Near Real-time Measurements
| dc.contributor.author | Kabandana, Giraso Keza Monia | |
| dc.contributor.author | Ratajczak, Adam Michael | |
| dc.contributor.author | Chen, Chengpeng | |
| dc.date.accessioned | 2020-11-04T16:59:02Z | |
| dc.date.available | 2020-11-04T16:59:02Z | |
| dc.date.issued | 2020-09-09 | |
| dc.description.abstract | Microfluidic technology has tremendously facilitated the development of in vitro cell cultures and studies. Conventionally, microfluidic devices are fabricated with extensive facilities by well-trained researchers, which hinders the widespread adoption of the technology for broader applications. Enlightened by the fact that low-cost microbore tubing is a natural microfluidic channel, we developed a series of adaptors in a toolkit that can twine, connect, organize, and configure the tubing to produce functional microfluidic units. Three subsets of the toolkit were thoroughly developed: the tubing and scoring tools, the flow adaptors, and the 3D cell culture suite. To demonstrate the usefulness and versatility of the toolkit, we assembled a microfluidic device and successfully applied it for 3D macrophage cultures, flow-based stimulation, and automated near real-time quantitation with new knowledge generated. Overall, we present a new technology that allows simple, fast, and robust assembly of customizable and scalable microfluidic devices with minimal facilities, which is broadly applicable to research that needs or could be enhanced by microfluidics. | en_US |
| dc.description.uri | https://figshare.com/articles/preprint/A_Modular_Toolkit_to_Fabricate_Microfluidic_Devices_for_3D_Cell_Culture_and_Near_Real-time_Measurements/12964604/1 | en_US |
| dc.format.extent | 18 pages | en_US |
| dc.genre | journal article preprints | en_US |
| dc.identifier | doi:10.13016/m2bjsv-wi9o | |
| dc.identifier.citation | Kabandana, Giraso; Ratajczak, Adam Michael; Chen, Chengpeng (2020): A Modular Toolkit to Fabricate Microfluidic Devices for 3D Cell Culture and Near Real-time Measurements. ChemRxiv. Preprint. https://doi.org/10.26434/chemrxiv.12964604.v1 | en_US |
| dc.identifier.uri | https://doi.org/10.26434/chemrxiv.12964604.v1 | |
| dc.identifier.uri | http://hdl.handle.net/11603/20010 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | figshare | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Chemistry & Biochemistry Department Collection | |
| dc.relation.ispartof | UMBC Faculty Collection | |
| dc.relation.ispartof | UMBC Student Collection | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
| dc.title | A Modular Toolkit to Fabricate Microfluidic Devices for 3D Cell Culture and Near Real-time Measurements | en_US |
| dc.type | Text | en_US |